Effects of botulinum toxin a on cytokine synthesis in a cell culture model of cutaneous scarring.

Archives of Facial Plastic Surgery Pub Date : 2012-03-01 Epub Date: 2011-10-17 DOI:10.1001/archfacial.2011.734

Frank Haubner, Elisabeth Ohmann, Uwe Müller-Vogt, Peter Kummer, Juergen Strutz, Holger G Gassner, Holger Gassner

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引用次数: 22

Abstract

Objective: To evaluate possible botulinum toxin A effects in a cell culture model.

Methods: In a cell culture model with dermal fibroblasts and microvascular endothelial cells, possible botulinum toxin A effects were evaluated. Cell proliferation and cytokine expression were analyzed using viability assays and enzyme-linked immunosorbent assay techniques.

Results: Neither cell proliferation nor cytokines and growth factors (interleukin 6, monocyte chemoattractant protein 2, fibroblast growth factor, macrophage colony-stimulating factor, and vascular endothelial growth factor) were affected by botulinum toxin A incubation.

Conclusions: The present data do not add evidence to suggest a significant therapeutic role of botulinum toxin A injections for cutaneous wound healing beyond chemoimmobilization. Further studies that include patient-specific cells of hypertrophic scars are required to better understand what role botulinum toxin A can play in the treatment of mature scar tissue.

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肉毒毒素a对皮肤瘢痕细胞培养模型中细胞因子合成的影响。

目的:评价A型肉毒毒素在细胞培养模型中的作用。方法:在真皮成纤维细胞和微血管内皮细胞的细胞培养模型中，评估肉毒毒素a可能的作用。采用活力测定和酶联免疫吸附测定技术分析细胞增殖和细胞因子表达。结果:A型肉毒杆菌毒素对细胞增殖和细胞因子及生长因子(白细胞介素6、单核细胞趋化蛋白2、成纤维细胞生长因子、巨噬细胞集落刺激因子、血管内皮生长因子)均无影响。结论:目前的数据并没有增加证据表明肉毒毒素a注射对皮肤伤口愈合的显著治疗作用，而不是化学固定。为了更好地了解肉毒杆菌毒素A在成熟疤痕组织治疗中的作用，需要对增生性疤痕患者特异性细胞进行进一步的研究。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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