Levels of 5-hydroxyindol acetic acid and lipid peroxidation in brain after administration of marijuana and nalbuphine in male and female rat.

Abstract

We analyzed the effect of marijuana and nalbuphine on levels of 5-hydroxyindol acetic acid and lipid peroxidation in rat brain. Single and repeated dosages of 250 mg/kg marijuana extract or 10 mg/kg nalbuphine were administered to male and female Wistar rats. Animals were sacrificed and brains were obtained to measure the content of 5-hydroxyindol acetic acid, reduced glutathione, thiobarbituric acid reactive substances, total ATPase and Na+/K+ ATPase activities. There was an increase in thiobarbituric acid reactive substances, total ATPase and Na+/K+ ATPase activity in the animals that received a single dose of marijuana and nalbuphine (p=0.001), with a notable decrease in glutathione and 5-hydroxyindol acetic acid levels (p=0.001). Both marijuana and nalbuphine increased levels of oxidative damage biomarkers in rat brain and decreased glutathione and 5-hydroxyindol acetic acid levels which could provoke changes in cellular and biochemical regulations and serotonergic activity in either male or female rats.