Heungbok Kim, Cecelia Webster, Justin K M Roberts, Juthamas Kositsawat, Li-Wei Hung, Thomas C Terwilliger, Chang-Yub Kim
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引用次数: 0
Abstract
Ligands interacting with Mycobacterium tuberculosis recombinant proteins were identified through use of the ability of Cibacron Blue F3GA dye to interact with nucleoside/nucleotide binding proteins, and the effects of these ligands on crystallization were examined. Co-crystallization with ligands enhanced crystallization and enabled X-ray diffraction data to be collected to a resolution of atleast 2.7 Å for 5 of 10 proteins tested. Additionally, clues about individual proteins’ functions were obtained from their interactions with each of a panel of ligands.
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