Crystallization and preliminary X-ray crystallographic analysis of the Bag2 amino-terminal domain from Mus musculus.

Richard C Page, Zhen Xu, Joseph Amick, Jay C Nix, Saurav Misra
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Abstract

Bag2, an atypical member of the Bag family of Hsp70 co-chaperones, acts as both an Hsp70 nucleotide-exchange factor and an inhibitor of the Hsp70-binding E3 ubiquitin ligase CHIP (carboxyl-terminus of Hsp70-interacting protein). The amino-terminal domain of Bag2 (Bag2-NTD), which is required for inhibition of CHIP, has no sequence homologs in the PDB. Native and selenomethionyl (SeMet) forms of Bag2-NTD were crystallized by hanging-drop vapor diffusion. Native Bag2-NTD crystals diffracted to 2.27 Å resolution and belonged to space group P2(1)2(1)2(1), with unit-cell parameters a = 75.5, b = 84.7, c = 114.1 Å. SeMet Bag2-NTD crystals diffracted to 3.10 Å resolution and belonged to space group P2(1)2(1)2(1), with unit-cell parameters a = 37.2, b = 53.3, c = 86.7 Å. Phases for the SeMet Bag2-NTD crystal were solved by single-wavelength anomalous diffraction. Initial phasing and model building using the 3.10 Å resolution SeMet Bag2-NTD data set suggested that Bag2-NTD forms a dimer and adopts a fold distinct from those of any domains annotated in the Pfam or SMART domain databases.

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麝香鹿 Bag2 氨基末端结构域的结晶和初步 X 射线晶体学分析。
Bag2是Hsp70辅助伴侣蛋白Bag家族的一个非典型成员,它既是Hsp70核苷酸交换因子,又是Hsp70结合型E3泛素连接酶CHIP(Hsp70相互作用蛋白的羧基端)的抑制剂。抑制 CHIP 所需的 Bag2 氨基末端结构域(Bag2-NTD)在 PDB 中没有同源序列。Bag2-NTD 的原生形式和硒蛋氨酰(SeMet)形式是通过悬滴蒸气扩散法结晶的。原生 Bag2-NTD 晶体的衍射分辨率为 2.27 Å,属于空间群 P2(1)2(1)2(1),单位晶胞参数 a = 75.5、b = 84.7、c = 114.1 Å。10 Å 分辨率,属于空间群 P2(1)2(1)2(1),单位胞参数 a = 37.2、b = 53.3、c = 86.7 Å。利用 3.10 Å 分辨率的 SeMet Bag2-NTD 数据集进行的初步分相和模型构建表明,Bag2-NTD 形成了一个二聚体,并采用了一种不同于 Pfam 或 SMART 结构域数据库中注释的任何结构域的折叠方式。
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期刊介绍: Acta Crystallographica Section F is a rapid structural biology communications journal. Articles on any aspect of structural biology, including structures determined using high-throughput methods or from iterative studies such as those used in the pharmaceutical industry, are welcomed by the journal. The journal offers the option of open access, and all communications benefit from unlimited free use of colour illustrations and no page charges. Authors are encouraged to submit multimedia content for publication with their articles. Acta Cryst. F has a dedicated online tool called publBio that is designed to make the preparation and submission of articles easier for authors.
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