Cloning, purification, crystallization and preliminary X-ray studies of human α1-microglobulin.

Yangli Zhang, Zengqiang Gao, Zhenzhen Zhang, Miao Luo, Ailong Huang, Yuhui Dong, Deqiang Wang
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Abstract

α(1)-Microglobulin (α(1)m) is one of the phylogenetically most widespread lipocalins and is distributed in various organs and tissues, including liver, heart, eye, kidney, brain, lung, pancreas and skeletal muscle. α(1)m has been found to exert multifarious functions, including interacting with IgA, albumin and prothrombin, binding strongly to haem and exhibiting reductase activity. Nevertheless, little structural information is available regarding these functions of α(1)m. Since determination of three-dimensional structure is a powerful means of functional characterization, X-ray crystallography was used to accomplish this task. Here, the expression, purification, crystallization and preliminary crystallographic analysis of human α(1)m are reported. The crystal belonged to space group P4(3), with unit-cell parameters a = b = 36.45, c = 112.68 Å, and diffracted to a resolution of 2.0 Å. The crystals are most likely to contain one molecule in the asymmetric unit, with a V(M) value of 1.63 Å(3) Da(-1).

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人 α1-微球蛋白的克隆、纯化、结晶和初步 X 射线研究。
α(1)-微球蛋白(α(1)m)是系统发育过程中分布最广的脂联素之一,分布于各种器官和组织,包括肝脏、心脏、眼睛、肾脏、大脑、肺、胰腺和骨骼肌。然而,有关 α(1)m 这些功能的结构信息却很少。由于三维结构的测定是功能表征的有力手段,因此 X 射线晶体学被用来完成这项任务。本文报道了人类α(1)m的表达、纯化、结晶和初步晶体学分析。该晶体属于空间群 P4(3),单位晶胞参数为 a = b = 36.45,c = 112.68 Å,衍射分辨率为 2.0 Å。
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期刊介绍: Acta Crystallographica Section F is a rapid structural biology communications journal. Articles on any aspect of structural biology, including structures determined using high-throughput methods or from iterative studies such as those used in the pharmaceutical industry, are welcomed by the journal. The journal offers the option of open access, and all communications benefit from unlimited free use of colour illustrations and no page charges. Authors are encouraged to submit multimedia content for publication with their articles. Acta Cryst. F has a dedicated online tool called publBio that is designed to make the preparation and submission of articles easier for authors.
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