Crystallization of the novel S-adenosyl-L-methionine-dependent C-methyltransferase CouO from Streptomyces rishiriensis and preliminary diffraction data analysis.
Andrzej Lyskowski, Martin Tengg, Georg Steinkellner, Helmut Schwab, Mandana Gruber-Khadjawi, Karl Gruber
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引用次数: 0
Abstract
Recombinant Q9F8T9 protein from Streptomyces rishiriensis (CouO), an S-adenosyl-L-methionine-dependent C-methyltransferase, has been successfully cloned, expressed and purified. CouO was crystallized from a single condition in the Morpheus crystallization screen. A vitrified crystal diffracted to 2.05 Å resolution and belonged to space group P2(1), with unit-cell parameters a = 33.02, b = 82.87, c = 76.77 Å, β = 96.93°.
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Acta Crystallographica Section F is a rapid structural biology communications journal.
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