Crystallization and preliminary crystallographic studies of both components of the staphylococcal LukE-LukD leukotoxin.

Romain Galy, Fabien Bergeret, Daniel Keller, Lionel Mourey, Gilles Prévost, Laurent Maveyraud
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引用次数: 4

Abstract

Soluble forms of recombinant LukE protein (expressed in Escherichia coli) and of wild-type LukD protein (expressed in Staphylococcus aureus), which together form the staphylococcal LukE-LukD leukotoxin, were purified to homogeneity and crystallized using the sitting-drop vapour-diffusion method. The crystals of LukE belonged to space group I4, with unit-cell parameters a = b = 134.50, c = 64.43 Å, and diffracted X-rays to 1.6 Å resolution. The crystals of LukD belonged to space group P2(1)2(1)2(1), with unit-cell parameters a = 48.04, b = 50.99, c = 137.40 Å, and diffracted to 1.9 Å resolution. Molecular replacement using the LukF-PV structure (PDB entry 1pvl) as a template model allowed the identification of an initial structure solution for the LukD data. In the case of LukE, a solution comprising only a single copy of the search model (LukS-PV; PDB entry 1t5r) was found, although the unit-cell parameters indicated that up to three molecules could be accommodated in the asymmetric unit.

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葡萄球菌LukE-LukD白毒素两种成分的结晶和初步结晶学研究。
重组LukE蛋白(在大肠杆菌中表达)和野生型LukD蛋白(在金黄色葡萄球菌中表达)的可溶性形式,它们一起形成葡萄球菌LukE-LukD白细胞毒素,使用坐滴蒸汽扩散法纯化至均匀并结晶。LukE的晶体属于空间群I4,晶胞参数a=b=134.50,c=64.43Å,衍射X射线的分辨率为1.6Å。LukD的晶体属于空间群P2(1)2(1)1(1),晶胞参数a=48.04,b=50.99,c=137.40Å,衍射分辨率为1.9Å。使用LukF-PV结构(PDB条目1pvl)作为模板模型的分子置换允许识别LukD数据的初始结构解决方案。在LukE的情况下,发现了仅包括搜索模型的单个副本的解决方案(LukS-PV;PDB条目1t5r),尽管晶胞参数表明在不对称单元中最多可以容纳三个分子。
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期刊介绍: Acta Crystallographica Section F is a rapid structural biology communications journal. Articles on any aspect of structural biology, including structures determined using high-throughput methods or from iterative studies such as those used in the pharmaceutical industry, are welcomed by the journal. The journal offers the option of open access, and all communications benefit from unlimited free use of colour illustrations and no page charges. Authors are encouraged to submit multimedia content for publication with their articles. Acta Cryst. F has a dedicated online tool called publBio that is designed to make the preparation and submission of articles easier for authors.
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