A validated HPLC method for the determination of eriocalyxin B in plasma and its application to pharmacokinetic studies.

Arzneimittel-Forschung-Drug Research Pub Date : 2012-12-01 Epub Date: 2012-11-15 DOI:10.1055/s-0032-1329986

Z Wang, Q Yuan, J-H Sun, Z Cui, H-Y Chen, H Wu, J-x Chen

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引用次数: 1

Abstract

The purpose of this study was to determine EriB in plasma by using the method of HPLC and collect the preclinical pharmacokinetic parameters of EriB.The analysis involved a simple liquid-liquid extraction. After making alkaline with NaOH, plasma was extracted with diethyl ether and the organic extract was then evaporated. From there, the residue was reconstituted in to the mobile phase. Chromatographic separation was achieved on the C18 column using acetonitrile and 0.1% triethylamine as mobile phase delivered at 1.0 ml/min. The UV detector wavelength was set at 233 nm. Standard curves were linear over the concentration range of 50-2 500 ng/ml.The mean predicted concentrations of the quality control (QC) samples deviated by less than 3% from the corresponding nominal values; the intra-assay and inter-assay precision of the assay were within 10% relative standard deviation. The extraction recovery of EriB was more than 80%.The developed method has been applied to the pharmacokinetic study of EriB in rats.

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高效液相色谱法测定血药浓度及其在药动学研究中的应用。

本研究采用高效液相色谱法测定血浆中EriB的含量，收集EriB的临床前药动学参数。分析包括简单的液-液萃取。用氢氧化钠碱性后，用乙醚提取血浆，然后蒸发有机提取物。从那里，剩余物被重组到流动相。在C18色谱柱上进行色谱分离，流动相为乙腈和0.1%三乙胺，流速为1.0 ml/min。紫外检测器波长为233nm。在50 ~ 2 500 ng/ml浓度范围内，标准曲线呈线性。质量控制(QC)样品的平均预测浓度与相应标称值偏差小于3%;测定的内、间精密度均在10%的相对标准偏差内。EriB的提取率在80%以上。该方法已应用于EriB在大鼠体内的药代动力学研究。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Arzneimittel-Forschung-Drug Research 医学-化学综合

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4-8 weeks