Prolactin gene expression in primary central nervous system tumors.

Journal of negative results in biomedicine Pub Date : 2013-01-14 DOI:10.1186/1477-5751-12-4

Graziella Alebrant Mendes, Júlia Fernanda Semmelmann Pereira-Lima, Maria Beatriz Kohek, Geraldine Trott, Marlise Di Domenico, Nelson Pires Ferreira, Miriam da Costa Oliveira

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引用次数: 9

Abstract

Background: Prolactin (PRL) is a hormone synthesized in both the pituitary gland and extrapituitary sites. It has been associated with the occurrence of neoplasms and, more recently, with central nervous system (CNS) neoplasms. The aim of this study was to evaluate prolactin expression in primary central nervous system tumors through quantitative real-time PCR and immunohistochemistry (IH).

Results: Patient mean age was 49.1 years (SD 15.43), and females accounted for 70% of the sample. The most frequent subtype of histological tumor was meningioma (61.5%), followed by glioblastoma (22.9%). Twenty cases (28.6%) showed prolactin expression by immunohistochemistry, most of them females (18 cases, 90%). Quantitative real-time PCR did not show any prolactin expression.

Conclusions: Despite the presence of prolactin expression by IH, the lack of its expression by quantitative real-time PCR indicates that its presence in primary tumors in CNS is not a reflex of local production.

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泌乳素基因在原发性中枢神经系统肿瘤中的表达。

背景:催乳素(PRL)是垂体和垂体外部位合成的一种激素。它与肿瘤的发生有关，最近也与中枢神经系统(CNS)肿瘤有关。本研究的目的是通过实时荧光定量PCR和免疫组织化学(IH)技术评估泌乳素在原发性中枢神经系统肿瘤中的表达。结果:患者平均年龄49.1岁(SD 15.43)，女性占70%。组织学肿瘤最常见的亚型是脑膜瘤(61.5%)，其次是胶质母细胞瘤(22.9%)。20例(28.6%)患者免疫组化结果显示催乳素表达，以女性居多(18例，90%)。实时荧光定量PCR未检测到催乳素的表达。结论:尽管IH中存在催乳素的表达，但实时荧光定量PCR检测显示其在中枢神经系统原发肿瘤中未表达，表明催乳素在中枢神经系统原发肿瘤中的存在并不是局部分泌的反射。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Journal of negative results in biomedicine

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