[Use of Amplicor HIV-1 DNA test v.1.5 in diagnosis of perinatal infection from HIV-1 in Cuba].

Q4 Medicine Revista Cubana de Medicina Tropical Pub Date : 2010-05-01
Liuber Y Machado Zaldivar, Madeline Blanco de Armas, Ana Luisa Lubián Caballero, Héctor M Díaz Torres
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引用次数: 0

Abstract

Introduction: HIV-1 proviral DNA amplification is the preferential method for HIV diagnosis infection in infants and it has been used in Cuba since 1992. AMPLICOR HIV-1 DNA kit is an in vitro qualitative assay for the detection of HIV-1 proviral DNA in the whole blood.

Objective: this paper showed the results of the use of this kit for the first time in Cuba for the perinatal diagnosis of HIV-1 infection.

Methods: three hundred forty six whole blood samples from children of HIV seropositive women were analyzed by the AMPLICOR HIV-1 DNA kit in the period 2005-2007.

Results: among the tested samples, six were positive, and 340 negative.

Conclusions: the assay was reproducible under the Cuban conditions and the achieved results made the diagnosis and follow up of children of HIV-1 seropositive mothers possible.

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[在古巴使用Amplicor HIV-1 DNA检测v.1.5诊断围产期HIV-1感染]。
HIV-1前病毒DNA扩增是诊断婴儿HIV感染的首选方法,自1992年在古巴开始使用。AMPLICOR HIV-1 DNA试剂盒是一种体外定性检测全血中HIV-1前病毒DNA的方法。目的:介绍该试剂盒在古巴首次用于围产期HIV-1感染诊断的结果。方法:采用AMPLICOR HIV-1 DNA检测试剂盒对2005-2007年HIV血清阳性妇女儿童的346份全血样本进行分析。结果:检测样品中阳性6份,阴性340份。结论:该试验在古巴条件下具有可重复性,所取得的结果使HIV-1血清阳性母亲的儿童的诊断和随访成为可能。
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来源期刊
Revista Cubana de Medicina Tropical
Revista Cubana de Medicina Tropical Medicine-Infectious Diseases
CiteScore
0.30
自引率
0.00%
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0
期刊介绍: La Revista Cubana de Medicina Tropical tiene la misión de publicar artículos científicos especializados en medicina tropical, microbiología, parasitología, epidemiología y otras especialidades afines. Se distribuye directamente por el editor a los suscriptores en formato impreso (ISSN 0375-0760). Está dirigida a profesionales y técnicos en el campo de la medicina tropical, microbiología, parasitología y epidemiología. Recibe contribuciones en idioma español, inglés y portugués sin distinción en el país de procedencia.
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