In this work, we investigated the structural and biological properties of soybean protein isolate (SPI) after 0–8 h hydrolyzation with cell envelope proteinase (CEP) extracted from Lactobacillus delbrueckii subsp. bulgaricus. CEP hydrolysis increased the β-sheet and red-shifted the fluorescence peak, while decreasing the α-helix, indicating the unfolding of soybean proteins. Increased surface hydrophobicity and fluorescence of the soybean protein hydrolysates were correlated with the increased hydrophobic amino acid (from 209.67 to 217.6 mg/100 g). CEP tended to hydrolyze the N- and C-terminal regions of sequences dominated by Gly and Leu, which enhanced the antioxidant activity of the SPHs (lowest IC50s value of ABTS•+ and hydroxyl radical scavenging activity were 0.324 ± 0.006 mg/mL and 0.365 ± 0.001 mg/mL after 4 h hydrolysis). Comparison with the database of bioactive peptides suggested various potential biological activities, including antioxidant activity, angiotensin-converting enzyme inhibitory activity and dipeptidyl peptidase-IV inhibitory activity. The study findings have theoretical significance for the development of CEP hydrolysis and novel bioactive soybean peptides.
公司名称 | 产品信息 | 采购帮参考价格 |
---|---|---|
阿拉丁 | Casein | ¥25.00~¥102863.90 |
索莱宝 | Phthalaldehyde (OPA) | ¥20.00~¥46904.00 |
上海源叶 | Anhydrous lithium chloride | ¥27.00~¥34049.00 |
上海源叶 | Coomassie Brilliant Blue R250 | ¥16.00~¥12397.98 |
索莱宝 | Protein molecular weight marker (11–245 kDa) | |
索莱宝 | l -serine | |
阿拉丁 | SDS-PAGE gel preparation kit | |
阿拉丁 | 1,1-diphenyl-2-picrhydrazyl (DPPH) | |
阿拉丁 | 2,2a?2-biazo-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) | |
Sigma | EMPOREa?¢ C18 solid phase extraction cartridge (7 mm/3 mL) |