Graphite-like carbon nitride-laden gold nanoparticles as signal amplification label for highly sensitive lateral flow immunoassay of 17β-estradiol

IF 9.8 1区农林科学 Q1 CHEMISTRY, APPLIED Food Chemistry Pub Date : 2021-06-15 Epub Date: 2021-01-11 DOI:10.1016/j.foodchem.2021.129001

Xiaolin Yao , Zonghan Wang , Man Zhao , Sijie Liu , Lihong Su , Leina Dou , Tao Li , Jianlong Wang , Daohong Zhang

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引用次数: 17

Abstract

Conventional gold nanoparticles-based lateral flow immunoassays (AuNPs-LFIA) lack sensitivity. In this work, we developed a graphite-like carbon nitride-laden AuNPs (g-C₃N₄@Au) assisted LFIA to improve sensitivity for 17β-estradiol (E2) in foods. g-C₃N₄ nanosheets were applied as carriers, because of their excellent chemical stability, large surface areas and low-cost, loading large numbers of AuNPs to amplify signals and improve the overall response of g-C₃N₄@Au-based LFIA (g-C₃N₄@Au-LFIA). The lowest visual limit of detection (vLOD) of E2 is 0.5 ng mL⁻¹ for g-C₃N₄@Au-LFIA, which exhibit a significantly three-fold improved analytical performance compared with that of AuNPs-LFIA. Additionally, this method was successfully used to the detection of E2 in four spiked food samples, offering a great potential of the g-C₃N₄@Au based LFIA for its application in food products.

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石墨样氮化碳负载金纳米颗粒作为17β-雌二醇高灵敏度侧流免疫分析的信号放大标记

传统的基于金纳米颗粒的横向流动免疫分析(AuNPs-LFIA)缺乏敏感性。在这项工作中，我们开发了一种类似石墨的氮化碳负载AuNPs (g-C3N4@Au)，帮助LFIA提高对食物中17β-雌二醇(E2)的敏感性。由于g-C3N4纳米片具有优异的化学稳定性、较大的表面积和低成本，因此被用作载体，可以装载大量的aunp来放大信号并提高g-C3N4@Au-based LFIA的整体响应(g-C3N4@Au-LFIA)。对于g-C3N4@Au-LFIA, E2的最低视觉检出限(vLOD)为0.5 ng mL−1，与AuNPs-LFIA相比，分析性能显著提高了3倍。此外，该方法成功地用于4种加标食品样品中E2的检测，为基于g-C3N4@Au的LFIA在食品中的应用提供了巨大的潜力。

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来源期刊

Food Chemistry 工程技术-食品科技

CiteScore

16.30

自引率

10.20%

发文量

3130

审稿时长

122 days

期刊介绍： Food Chemistry publishes original research papers dealing with the advancement of the chemistry and biochemistry of foods or the analytical methods/ approach used. All papers should focus on the novelty of the research carried out.