Biochemical and biological characterization of Naja kaouthia venom from North-East India and its neutralization by polyvalent antivenom.

Journal of Venom Research Pub Date : 2013-11-06 eCollection Date: 2013-01-01

Diganta Das, Nanjaraj Urs, Vilas Hiremath, Bannikuppe Sannanaik Vishwanath, Robin Doley

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Abstract

This study describes biochemical and biological properties of Naja kaouthia (Indian monocled cobra) venom of North-East India. The LD50 of the crude venom was found to be 0.148mg/kg and neurotoxicitic symptoms like paralysis of lower limbs and heavy difficulty in breathing at sub-lethal dose in mice was observed. The venom exhibited PLA2, indirect hemolytic and myotoxic activities but showed weak proteolytic and low direct hemolytic activities. It did not exhibit any hemorrhage when injected intradermally to mice. Anticoagulant activity was prominent when recalcification, prothrombin and activated partial thrombinplastin time were tested on platelet poor plasma. Rotem analysis of whole citrated blood in presence of venom showed delay in coagulation time and clot formation time. Fibrinogen of whole citrated blood was depleted by venom when analyzed in Sonoclot. Crude venom at 10µg and after 16hr of incubation was found to degrade α chain of fibrinogen. Neutralization study showed that Indian polyvalent antivenom could neutralize some of the biochemical and biological activities as well as its fibrinogenolytic activity.

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产自印度东北部的蛇舌蛇毒的生化生物学特性及其多价抗蛇毒血清的中和作用。

本研究描述了印度东北部单目眼镜蛇(Naja kaouthia)毒液的生化和生物学特性。粗毒的LD50为0.148mg/kg，亚致死剂量小鼠出现下肢瘫痪、呼吸严重困难等神经毒性症状。该毒液具有PLA2、间接溶血和肌毒活性，但具有较弱的蛋白溶解和较低的直接溶血活性。经皮内注射小鼠未见出血。在血小板差的血浆中检测再钙化、凝血酶原和活化部分凝血酶活时间时，抗凝活性显著。柠檬酸全血在毒液作用下的凝血时间和凝块形成时间延迟。全柠檬酸血纤维蛋白原在索诺克洛分析时被毒液耗尽。粗毒液在10µg孵育16h后可降解纤维蛋白原α链。中和研究表明，印度多价抗蛇毒血清能中和部分生物生化活性及纤维蛋白原溶解活性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Journal of Venom Research

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