Cryoglobulins as potential triggers of inflammation in schizophrenia.

IF 3.6 Q1 PSYCHIATRY Schizophrenia Research and Treatment Pub Date : 2013-01-01 Epub Date: 2013-12-15 DOI:10.1155/2013/125264
Andranik Chavushyan, Meri Hovsepyan, Anna Boyajyan
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引用次数: 3

Abstract

This case study aimed to investigate effects of type III cryoglobulins isolated from the blood of patients with schizophrenia on the production of proinflammatory cytokines interleukin(IL)-1 β , IL-6 and tumor necrosis factor- α (TNF- α ), anti-inflammatory cytokine IL-10, and chemotactic cytokines IL-8 and monocyte chemoattractant protein-1 (MCP-1) by peripheral blood mononuclear cells (PBMCs). The experiments were performed in vitro using PBMCs healthy subjects and the blood of patients whit schizoprenia. The enzyme-linked immunosorbent assay and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay were used upon study. The results obtained indicated significant increase (P < 0.05) in IL-1 β , IL-6, TNF- α , IL-8, and MCP-1 production by cultured PBMCs when incubating for 24 hours with cryoglobulins, beginning from 0.4 mg/mL. The gender difference does not affect the cryoglobulins-induced production of these cytokines by PBMCs. No influence of cryoglobulins on production of IL-10 by PBMCs was observed. Also, it was shown that cryoglobulins in concentration ≤4 mg/mL possessed no cytotoxic effect towards cultured PBMCs. Based upon the results obtained, we concluded that type III cryoglobulins are implicated in schizophrenia-associated alterations in the immune response through induction of the expression of proinflammatory and chemotactic cytokines by PBMCs.

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低温球蛋白作为精神分裂症炎症的潜在诱因。
本研究旨在探讨从精神分裂症患者血液中分离的III型冷球蛋白对外周血单核细胞(PBMCs)产生促炎细胞因子白介素(IL)-1 β、IL-6和肿瘤坏死因子- α (TNF- α)、抗炎细胞因子IL-10、趋化细胞因子IL-8和单核细胞趋化蛋白-1 (MCP-1)的影响。实验采用pbmc健康受试者和精神分裂症患者的血液进行体外实验。采用酶联免疫吸附法和3-(4,5-二甲基噻唑-2-基)-2,5-二苯基溴化四唑测定法。结果表明,从0.4 mg/mL开始,低温球蛋白孵育24小时后,培养的pbmc细胞IL-1 β、IL-6、TNF- α、IL-8和MCP-1的产量显著增加(P < 0.05)。性别差异不影响冷球蛋白诱导pbmc产生这些细胞因子。未观察到低温球蛋白对PBMCs产生IL-10的影响。低温球蛋白浓度≤4 mg/mL对培养的PBMCs无细胞毒作用。基于所获得的结果,我们得出结论,III型冷球蛋白通过诱导促炎和趋化细胞因子的表达,与精神分裂症相关的免疫反应改变有关。
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来源期刊
CiteScore
4.60
自引率
0.00%
发文量
2
审稿时长
14 weeks
期刊介绍: Schizophrenia Research and Treatment is a peer-reviewed, Open Access journal that publishes original research articles, review articles, and clinical studies related to all aspects of schizophrenia.
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