Central Role of the PPARγ Gene Network in Coordinating Beef Cattle Intramuscular Adipogenesis in Response to Weaning Age and Nutrition.

Gene regulation and systems biology Pub Date : 2014-01-08 eCollection Date: 2014-01-01 DOI:10.4137/GRSB.S11782
Sonia J Moisá, Daniel W Shike, Dan B Faulkner, William T Meteer, Duane Keisler, Juan J Loor
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引用次数: 50

Abstract

Adipogenic/lipogenic transcriptional networks regulating intramuscular fat deposition (IMF) in response to weaning age and dietary starch level were studied. The longissimus muscle (LM) of beef steers on an early weaning (141 days age) plus high-starch diet (EWS) or a normal weaning (NW, 222 days age) plus starch creep-feed diet (CFS) was biopsied at 0 (EW), 25, 50, 96 (NW), 167, and 222 (pre-slaughter) days. Expression patterns of 35 target genes were studied. From NW through slaughter, all steers received the same high-starch diet. In EWS steers the expression of PPARG, other adipogenic (CEBPA, ZFP423) and lipogenic (THRSP, SREBF1, INSIG1) activators, and several enzymes (FASN, SCD, ELOVL6, PCK1, DGAT2) that participate in the process of IMF increased gradually to a peak between 96 and 167 days on treatment. Steers in NW did not achieve similar expression levels even by 222 days on treatment, suggesting a blunted response even when fed a high-starch diet after weaning. High-starch feeding at an early age (EWS) triggers precocious and sustained adipogenesis, resulting in greater marbling.

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PPARγ基因网络在协调肉牛对断奶日龄和营养的肌内脂肪形成中的核心作用
研究了断奶日龄和饲粮淀粉水平对肌内脂肪沉积(IMF)调控的成脂/成脂转录网络。分别在0 (EW)、25、50、96 (NW)、167和222(屠宰前)天对早期断奶(141日龄)加高淀粉饲粮(EWS)或正常断奶(NW, 222日龄)加淀粉匍匐饲粮(CFS)的肉牛最长肌(LM)进行活组织检查。研究了35个靶基因的表达模式。从西北至屠宰,所有阉牛均饲喂相同的高淀粉日粮。在EWS小鼠中,参与IMF过程的PPARG、其他致脂因子(CEBPA、ZFP423)和致脂因子(THRSP、SREBF1、INSIG1)以及几种酶(FASN、SCD、ELOVL6、PCK1、DGAT2)的表达在治疗后96 ~ 167天逐渐增加,达到峰值。西北地区的阉牛即使在治疗222天后也没有达到类似的表达水平,这表明即使在断奶后喂食高淀粉饮食,它们的反应也会变迟钝。早期高淀粉喂养(EWS)会引发早熟和持续的脂肪生成,导致更大的大理石纹。
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