Ageratum conyzoides attenuates alcohol induced liver toxicity in male Wistar rats.

Nigerian quarterly journal of hospital medicine Pub Date : 2013-01-01

B Ogunlade, O E Yama, L C Saalu, A B Clement

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Abstract

Background: Ageratum conyzoides (AC) is unique for its antioxidant activity and protective role to tissues. However this property is yet to be demonstrated in animals administered toxic alcohol concentrations.

Objective: To determine the effect of AC extract on the oxidative stress, liver enzymes and histology.

Methods: Twenty four male rats (190-230 g) were divided into three groups of eight rats. Group A (control) administered distilled water. Group B (ethanol group) received 10 g/kg body weight of ethanol. Group C (ethanol + AC group) were treated with ethanol (as above) and AC (250 mg/kg body weight) concurrently. Total experimental duration was 35 days at the end of which animals were euthanized by cervical dislocation. Liver and blood samples were taken and processed for: microscopic studies, estimation of activities of liver enzymes [alanine aminotransferase (AST), aspartate aminotransferase (ALT) and alkaline phosphatase (ALP)], Malondialdehyde (MDA) and antioxidants [Superoxide dismutase (SOD), Glutathione peroxidase (GSH-Px) and Catalase (CAT)] respectively.

Results: Compared to control, the rats treated with ethanol had significantly increased liver enzymes and MDA levels but these were decreased in ethanol + AC group compared to the ethanol group. The histologies of concurrent ethanol + AC treated group were similar to control groups.

Conclusion: AC protects the liver against alcohol induced damage.

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鹰嘴藤可减轻酒精诱导的雄性Wistar大鼠肝毒性。

背景:延胡结(Ageratum conyzoides, AC)具有独特的抗氧化活性和组织保护作用。然而，这一特性尚未在给予有毒酒精浓度的动物身上得到证实。目的:探讨AC提取物对大鼠氧化应激、肝酶及肝组织的影响。方法:雄性大鼠24只，体重190 ~ 230 g，随机分为3组，每组8只。A组(对照组)给予蒸馏水。B组(乙醇组)给予乙醇10 g/kg体重。C组(乙醇+ AC组)采用乙醇(同上)和AC (250 mg/kg体重)同时处理。实验总时间为35 d，实验结束后采用颈椎脱位法安乐死。取肝脏和血液标本，分别进行显微镜观察、肝酶[谷丙转氨酶(AST)、天冬氨酸转氨酶(ALT)和碱性磷酸酶(ALP)]、丙二醛(MDA)和抗氧化剂[超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)和过氧化氢酶(CAT)]活性测定。结果:与对照组相比，乙醇处理大鼠肝酶和MDA水平显著升高，而乙醇+ AC组较乙醇组降低。乙醇+ AC同时处理组的组织学与对照组相似。结论:AC具有保护肝脏免受酒精性损伤的作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Nigerian quarterly journal of hospital medicine

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