A microsurgical procedure for middle cerebral artery occlusion by intraluminal monofilament insertion technique in the rat: a special emphasis on the methodology.

Experimental & Translational Stroke Medicine Pub Date : 2014-06-06 eCollection Date: 2014-01-01 DOI:10.1186/2040-7378-6-6
Aslan Güzel, Roland Rölz, Guido Nikkhah, Ulf D Kahlert, Jaroslaw Maciaczyk
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引用次数: 22

Abstract

Introduction: Although there are many experimental studies describing the methodology of the middle cerebral artery occlusion (MCAO) in the literature, only limited data on these distinct anatomical structures and the details of the surgical procedure in a step by step manner. The aim of the present study simply is to examine the surgical anatomy of MCAO model and its modifications in the rat.

Materials and methods: Forty Sprague-Dawley rats were used; 20 during the training phase and 20 for the main study. The monofilament sutures were prepared as described in the literature. All surgical steps of the study were performed under the operating microscope, including insertion of monofilament into middle cerebral artery through the internal carotid artery.

Results: After an extensive training period, we lost two rats in four weeks. The effects of MCAO were confirmed by the evidence of severe motor deficit during the recovery period, and histopathological findings of infarction were proved in all 18 surviving rats.

Conclusion: In this study, a microsurgical guideline of the MCAO model in the rat is provided with the detailed description of all steps of the intraluminal monofilament insertion method with related figures.

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用腔内单丝插入技术治疗大鼠大脑中动脉闭塞的显微外科手术:特别强调方法。
虽然文献中有许多实验研究描述了大脑中动脉闭塞(MCAO)的方法学,但对这些独特的解剖结构和循序渐进的手术过程细节的数据有限。本研究的目的仅仅是研究MCAO模型的手术解剖及其在大鼠中的修饰。材料与方法:选取Sprague-Dawley大鼠40只;训练阶段20人,主要学习阶段20人。单丝缝线按文献所述制备。本研究的所有手术步骤均在手术显微镜下完成,包括通过颈内动脉将单丝插入大脑中动脉。结果:经过长时间的训练,我们在四周内失去了两只大鼠。18只存活的大鼠在恢复期出现严重的运动障碍,证实了MCAO的作用,并证实了梗死的组织病理学结果。结论:本研究提供了一份大鼠MCAO模型的显微外科指南,详细描述了腔内单丝插入方法的各个步骤并附有相关图。
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