An improved experimental model of hemorrhoids in rats: evaluation of antihemorrhoidal activity of an herbal formulation.

ISRN Pharmacology Pub Date : 2014-03-11 eCollection Date: 2014-01-01 DOI:10.1155/2014/530931
Mohammed Azeemuddin, Gollapalle Lakshminarayanashastry Viswanatha, Mohamed Rafiq, Agadi HireMath Thippeswamy, Mirza Rizwan Baig, Kethaganahalli Jayaramaiah Kavya, Pralhad Sadashiv Patki, Ramakrishnan Shyam
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引用次数: 33

Abstract

Objective. To improve the existing experimental model of croton oil-induced hemorrhoids in rats by using Evans Blue (EB) dye extravasation technique. Further, an herbal formulation (Pilex) was evaluated for its antihemorrhoidal activity in this model. Methods. Two sets of experiments were carried out: first to improve the experimental model and to validate the same using Pilex and second to evaluate the effect of Pilex on cytoarchitecture of rectoanal tissue in croton oil-induced hemorrhoids. In both sets, hemorrhoids were induced to all the animals, except normal controls, by applying croton oil via rectoanal region and the effect of Pilex ointment (PO), Pilex granules (PG), and combination of PG and PO was evaluated. In the first set, extravasation of EB dye, TNF- α , IL-6, and rectoanal coefficient (RAC) was determined. In the second set, severity of score, RAC, and histopathology were evaluated. Results. The elevated levels of TNF- α , IL-6, and extravasations of EB dye were decreased with the Pilex treatment. The cytoarchitecture of rectoanal portion of the animals treated with Pilex was near to normal. Conclusion. The improved experimental model of hemorrhoid is useful in quantifying the inflammatory exudates and extent of inflammation. In this improved experimental model Pilex showed antihemorrhoidal activity, which further validates its clinical usage.

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改进的大鼠痔疮实验模型:中药制剂抗痔疮活性的评价。
目标。目的:采用Evans Blue (EB)染料外渗技术,改进现有的大鼠豆油性痔疮实验模型。此外,在该模型中评估了一种草药制剂(Pilex)的抗痔疮活性。方法。本研究进行了两组实验:一是对实验模型进行改进,并用Pilex进行验证;二是评价Pilex对豆油致痔疮直肠组织细胞结构的影响。两组大鼠除正常对照组外,均采用巴豆油经肛区灌胃法诱导大鼠痔疮,并观察皮毛软膏(PO)、皮毛颗粒(PG)及皮毛颗粒与皮毛颗粒联合用药的效果。在第一组中,测定EB染料外渗、TNF- α、IL-6和直肠系数(RAC)。在第二组中,评估评分的严重程度、RAC和组织病理学。结果。Pilex处理降低了TNF- α、IL-6升高水平和EB染料外渗。经皮莱克斯处理的动物直肠部分的细胞结构接近正常。结论。改进后的痔疮实验模型有助于量化炎症渗出物和炎症程度。在改进的实验模型中,Pilex显示出抗痔疮活性,进一步验证了其临床应用价值。
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