Comparative analysis of diosgenin in Dioscorea species and related medicinal plants by UPLC-DAD-MS.

Q2 Biochemistry, Genetics and Molecular Biology BMC Biochemistry Pub Date : 2014-08-09 DOI:10.1186/1471-2091-15-19

Tao Yi, Lan-Lan Fan, Hong-Li Chen, Guo-Yuan Zhu, Hau-Man Suen, Yi-Na Tang, Lin Zhu, Chu Chu, Zhong-Zhen Zhao, Hu-Biao Chen

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引用次数: 69

Abstract

Background: Dioscorea is a genus of flowering plants, and some Dioscorea species are known and used as a source for the steroidal sapogenin diosgenin. To screen potential resource from Dioscorea species and related medicinal plants for diosgenin extraction, a rapid method to compare the contents of diosgenin in various plants is crucial.

Results: An ultra-performance liquid chromatography (UPLC) coupled with diode array detection (DAD) and electrospray ionization mass spectrometry (ESI-MS) method was developed for identification and determination of diosgenin in various plants. A comprehensive validation of the developed method was conducted. Twenty-four batches of plant samples from four Dioscorea species, one Smilax species and two Heterosmilax species were analyzed by using the developed method.The present method presented good sensitivity, precision and accuracy. Diosgenin was found in three Dioscorea species and one Heterosmilax species, namely D. zingiberensis, D. septemloba, D. collettii and H. yunnanensis.

Conclusion: The method is suitable for the screening of diosgenin resources from plants. D. zingiberensis is an important resource for diosgenin harvesting.

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UPLC-DAD-MS分析薯蓣属植物与相关药用植物中薯蓣皂苷元的比较。

背景:薯蓣属是一种开花植物属，一些薯蓣属植物被认为是甾体皂苷元薯蓣皂苷元的来源。为了从薯蓣属植物及相关药用植物中筛选提取薯蓣皂苷元的潜在资源，建立一种快速比较不同植物中薯蓣皂苷元含量的方法至关重要。结果:建立了超高效液相色谱(UPLC) -二极管阵列检测(DAD) -电喷雾电离质谱(ESI-MS)联用方法，用于多种植物中薯蓣皂苷元的鉴别和测定。对所建立的方法进行了综合验证。采用该方法对4种薯蓣属植物、1种菝葜属植物和2种杂花属植物的24批样品进行了分析。该方法具有良好的灵敏度、精密度和准确度。在3种薯蓣属植物和1种异种薯蓣属植物中均发现薯蓣皂苷元，分别为盾叶薯蓣、九头薯蓣、collettii薯蓣和云南薯蓣。结论:该方法适用于植物薯蓣皂苷元资源的筛选。盾叶薯蓣是薯蓣皂苷元的重要资源。

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来源期刊

BMC Biochemistry BIOCHEMISTRY & MOLECULAR BIOLOGY-

CiteScore

4.80

自引率

0.00%

发文量

审稿时长

3 months

期刊介绍： BMC Biochemistry is an open access journal publishing original peer-reviewed research articles in all aspects of biochemical processes, including the structure, function and dynamics of metabolic pathways, supramolecular complexes, enzymes, proteins, nucleic acids and small molecular components of organelles, cells and tissues. BMC Biochemistry (ISSN 1471-2091) is indexed/tracked/covered by PubMed, MEDLINE, BIOSIS, CAS, EMBASE, Scopus, Zoological Record, Thomson Reuters (ISI) and Google Scholar.