Inhibitory potential of methanolic extracts of Aristolochia tagala and Curcuma caesia on hepatocellular carcinoma induced by diethylnitrosamine in BALB/c mice.
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Abstract
Context: Aristolochia tagala (AT) and Curcuma caesia (CC) have been used traditionally by local herbal practitioners for cancer treatment and as chief ingredients of many polyherbal formulations for various types of ailments. However, there is void in scientific study to evaluate their anti-cancer property.
Aims: The aim of this study was to evaluate the anti-carcinogenic properties of the crude methanolic extracts of roots of AT and rhizomes of CC in BALB/c mice exposed to a hepatocarcinogen, diethylnitrosamine (DEN).
Settings and design: (I) Toxicity of herbal plant extracts (HPE); (II) Anticancer studies; (III) Histological studies; and (IV) Biochemical studies.
Materials and methods: To evaluate the effects of these two HPE either alone or following DEN exposure, serum transaminases (aspartate aminotransferase [AST], alanine aminotransferase [ALT]), alkaline phosphatase (ALP), and cancer marker enzyme acetylcholine esterase (AChE) were assayed in mice. In addition, histological study was also carried out under similar conditions. The antioxidant potentials of the HPE were evaluated by monitoring the activity of antioxidant enzymes and metabolites, such as superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), and glutathione (GSH).
Statistical analysis used: Statistical analysis was performed by GraphPad Prism 6 Software using one-way analysis of variance followed by the Tukey's multiple comparisons test. Significance was set at P < 0.05.
Results: Our findings show that DEN administration elevated AST, ALT, ALP, and AChE activities. CC or AT extracts attenuated the increased activities of these marker enzymes. The activities of antioxidant enzymes, which were decreased following DEN administration, were significantly increased in mice treated with CC or AT.
Conclusions: The present study clearly documents anticarcinogenic and antioxidant properties of AT and CC in DEN-induced mouse liver cancer in vivo.
背景：马兜铃（AT）和莪术（CC）一直被当地草药医师用于治疗癌症，也是许多治疗各种疾病的多草药配方的主要成分。目的：本研究旨在评估暴露于肝致癌物二乙基亚硝胺（DEN）的 BALB/c 小鼠体内的 AT 根和 CC 根茎粗甲醇提取物的抗癌特性。设置和设计：（I）草本植物提取物（HPE）的毒性；（II）抗癌研究；（III）组织学研究；以及（IV）生化研究：为了评估这两种草本植物提取物单独或暴露于 DEN 后的影响，对小鼠的血清转氨酶（天冬氨酸氨基转移酶 [AST]、丙氨酸氨基转移酶 [ALT]）、碱性磷酸酶（ALP）和癌症标志酶乙酰胆碱酯酶（AChE）进行了检测。此外，还在类似条件下进行了组织学研究。通过监测超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、谷胱甘肽过氧化物酶（GPx）和谷胱甘肽（GSH）等抗氧化酶和代谢物的活性，评估了 HPE 的抗氧化潜力：使用 GraphPad Prism 6 软件进行统计分析，采用单因素方差分析和 Tukey's 多重比较检验。显著性以 P < 0.05 为标准：我们的研究结果表明，服用 DEN 会升高 AST、ALT、ALP 和 AChE 活性。CC或AT提取物可减轻这些标记酶活性的升高。抗氧化酶的活性在服用 DEN 后降低，而在使用 CC 或 AT 治疗的小鼠中则明显提高：本研究清楚地证明了 AT 和 CC 在 DEN 诱导的体内小鼠肝癌中的抗癌和抗氧化特性。
期刊介绍：
Journal of Carcinogenesis considers manuscripts in many areas of carcinogenesis and Chemoprevention. Primary areas of interest to the journal include: physical and chemical carcinogenesis and mutagenesis; processes influencing or modulating carcinogenesis, such as DNA repair; genetics, nutrition, and metabolism of carcinogens; the mechanism of action of carcinogens and modulating agents; epidemiological studies; and, the formation, detection, identification, and quantification of environmental carcinogens. Manuscripts that contribute to the understanding of cancer prevention are especially encouraged for submission
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