Matrix gla protein binds to fibronectin and enhances cell attachment and spreading on fibronectin.

Q3 Biochemistry, Genetics and Molecular Biology International Journal of Cell Biology Pub Date : 2014-01-01 Epub Date: 2014-08-21 DOI:10.1155/2014/807013
Satoru Ken Nishimoto, Miyako Nishimoto
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引用次数: 17

Abstract

Background. Matrix Gla protein (MGP) is a vitamin K-dependent, extracellular matrix protein. MGP is a calcification inhibitor of arteries and cartilage. However MGP is synthesized in many tissues and is especially enriched in embryonic tissues and in cancer cells. The presence of MGP in those instances does not correlate well with the calcification inhibitory role. This study explores a potential mechanism for MGP to bind to matrix proteins and alter cell matrix interactions. Methods. To determine whether MGP influences cell behavior through interaction with fibronectin, we studied MGP binding to fibronectin, the effect of MGP on fibronectin mediated cell attachment and spreading and immunolocalized MGP and fibronectin. Results. First, MGP binds to fibronectin. The binding site for MGP is in a specific fibronectin fragment, called III1-C or anastellin. The binding site for fibronectin is in a MGP C-terminal peptide comprising amino acids 61-77. Second, MGP enhances cell attachment and cell spreading on fibronectin. MGP alone does not promote cell adhesion. Third, MGP is present in fibronectin-rich regions of tissue sections. Conclusions. MGP binds to fibronectin. The presence of MGP increased cell-fibronectin interactions.

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基质玻璃蛋白与纤维连接蛋白结合,增强细胞在纤维连接蛋白上的附着和扩散。
背景。基质玻璃蛋白(MGP)是一种依赖维生素k的细胞外基质蛋白。MGP是动脉和软骨的钙化抑制剂。然而,MGP在许多组织中合成,尤其是在胚胎组织和癌细胞中富集。在这些情况下,MGP的存在与钙化抑制作用并不相关。本研究探讨了MGP与基质蛋白结合并改变细胞基质相互作用的潜在机制。方法。为了确定MGP是否通过与纤维连接蛋白的相互作用影响细胞行为,我们研究了MGP与纤维连接蛋白的结合、MGP对纤维连接蛋白介导的细胞附着和扩散的影响以及MGP和纤维连接蛋白的免疫定位。结果。首先,MGP与纤维连接蛋白结合。MGP的结合位点在一个特定的纤维连接蛋白片段中,称为III1-C或anastellin。纤维连接蛋白的结合位点在MGP c端肽中,包含61-77个氨基酸。其次,MGP增强纤维连接蛋白上的细胞附着和细胞扩散。MGP单独不促进细胞粘附。第三,MGP存在于组织切片中富含纤维连接蛋白的区域。结论。MGP与纤维连接蛋白结合。MGP的存在增加了细胞-纤维连接蛋白的相互作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
International Journal of Cell Biology
International Journal of Cell Biology Biochemistry, Genetics and Molecular Biology-Cell Biology
CiteScore
3.30
自引率
0.00%
发文量
4
审稿时长
20 weeks
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