Enterococcus--virulence and susceptibility to photodynamic therapy of clinical isolates from Lower Silesia, Poland.

Ewa Dworniczek, Justyna Piwowarczyk, Alicja Seniuk, Grażyna Gościniak
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引用次数: 10

Abstract

Background: A collection of 400 enterococcal isolates from clinical samples of hospitalized patients were studied for their virulence traits according to the isolation site. Formation of biofilm and production of DNase, hemolysin, lipase, and gelatinase were characterized. Biofilms of selected strains were examined for their susceptibility to antimicrobial photodynamic therapy (aPDT).

Methods: All strains were tested for biofilm production by microtiter method and the activity of hemolysin, gelatinase, lipase, and DNase by plate method with an adequate substrate. Photodynamic therapy with Photolon and red laser light was performed towards 48 h biofilms of eight representative strains. The viability of biofilms was tested by the BactLight assay and visualized under a fluorescent microscope.

Results: Among the 400 isolates, 69.8% strains of Enterococcus faecalis, 30% of Enterococcus faecium, and 0.2% of Enterococcus casseliflavus were identified. In vitro, production of biofilm was found in 65.7% of enterococci. Biofilm-positive strains were isolated from urinary tract infections (81%), wound infections (71%), respiratory tract infections (62%), and gastrointestinal tract (47%) (colonization). Hemolysin activity was observed in 28.5%, gelatinase in 24.5%, lipase in 23%, and DNase in 3.5% of all, mostly biofilm-positive, isolates. Photodynamic therapy with Photolon efficiently reduced the enterococcal biofilms.

Conclusions: The study demonstrated the high prevalence of biofilm-producing clinical enterococci, their virulence potential being higher than for biofilm-negative strains, and the susceptibility to aPDT of mature biofilms produced by strains, regardless of their species and site of isolation.

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肠球菌——波兰下西里西亚临床分离株的毒力和光动力治疗敏感性
背景:从住院患者临床标本中分离出400株肠球菌,根据分离地点对其毒力特征进行研究。研究了生物膜的形成和dna酶、溶血酶、脂肪酶和明胶酶的产生。对所选菌株的生物膜进行抗菌光动力治疗(aPDT)敏感性检测。方法:采用微滴法检测各菌株的生物膜产量,用平板法检测溶血素、明胶酶、脂肪酶和dna酶的活性。用光子和红色激光对8株代表性菌株的48 h生物膜进行光动力治疗。采用BactLight法检测生物膜的活力,并在荧光显微镜下观察。结果:400株分离物中检出粪肠球菌69.8%,粪肠球菌30%,casseliflavus肠球菌0.2%。在体外,65.7%的肠球菌产生生物膜。从尿路感染(81%)、伤口感染(71%)、呼吸道感染(62%)和胃肠道(47%)(定植)中分离出生物膜阳性菌株。在所有生物膜阳性的分离株中,溶血素活性为28.5%,明胶酶活性为24.5%,脂肪酶活性为23%,dna酶活性为3.5%。光动力疗法可有效减少肠球菌生物膜。结论:该研究表明,临床产生物膜肠球菌的流行率高,其毒力潜力高于生物膜阴性菌株,并且菌株产生的成熟生物膜对aPDT的敏感性,无论其种类和分离地点如何。
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