Molecular characteristics of rotavirus isolated from a diarrhea outbreak in october 2008 in bintuni bay, papua, indonesia.

Q1 Medicine Virology: Research and Treatment Pub Date : 2014-03-09 eCollection Date: 2014-01-01 DOI:10.4137/VRT.S13555

Eka Pratiwi, Vivi Setiawaty, Rudi Hendro Putranto

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引用次数: 7

Abstract

Background: Viral diarrhea continues to be a health problem in Indonesia that often causes outbreaks; in particular, acute viral diarrhea in young children. Rotavirus is the leading cause of severe diarrhea in children under two years of age. This study aimed to determine the genotypes of rotavirus in Bintuni Bay, Papua.

Methods: Stool specimens from 15 patients were collected and analyzed for rotavirus using an enzyme immunosorbent assay (EIA) and reverse transcriptase-polymerase chain reaction (RT-PCR). Subsequently, we sequenced the genetic material of rotavirus positive samples by RT-PCR and analyzed the results using Mega-4 software.

Results: Two rotavirus serotypes were identified from the diarrhea outbreak in Bintuni, Papua in October 2008: serotype G1 with G1P[6] (50%) and G1P[8] (16.7%) strains, and serotype G2 with G2P[4] (23.3%) strain. Phylogenetic tree analyses of VP7 protein showed that rotavirus-infected diarrhea in Bintuni Bay, Papua at that time was dominated by the G1 serotype (83%).

Conclusion: The laboratory results showed that G1 serotype rotavirus was a cause of the outbreak of diarrhea in October 2008 in Bintuni, Papua.

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2008年10月印度尼西亚巴布亚宾图尼湾腹泻暴发分离的轮状病毒的分子特征。

背景:病毒性腹泻仍然是印度尼西亚的一个健康问题，经常引起疫情;特别是幼儿急性病毒性腹泻。轮状病毒是导致两岁以下儿童严重腹泻的主要原因。本研究旨在确定巴布亚Bintuni湾轮状病毒的基因型。方法:收集15例患者粪便标本，采用酶免疫吸附试验(EIA)和逆转录聚合酶链反应(RT-PCR)对轮状病毒进行检测。随后，我们采用RT-PCR对轮状病毒阳性样本的遗传物质进行测序，并使用Mega-4软件对结果进行分析。结果:从2008年10月巴布亚Bintuni腹泻暴发中鉴定出两种轮状病毒血清型:G1血清型为G1P[6](50%)和G1P[8](16.7%)株，G2血清型为G2P[4](23.3%)株。VP7蛋白系统进化树分析显示，当时巴布亚Bintuni湾轮状病毒感染的腹泻以G1血清型为主(83%)。结论:实验室结果显示G1血清型轮状病毒是2008年10月在巴布亚宾图尼暴发腹泻的一个原因。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Virology: Research and Treatment Medicine-Infectious Diseases

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