Identification of species Leucochloridium paradoxum and L. perturbatum (Trematoda) based on rDNA sequences.

Parazitologiia Pub Date : 2014-05-01
A Zhukova, E E Prokhorova, A S Tokmakova, N V Tsymbalenko, G L Ataev
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Abstract

The full nucleotide sequences of DNA ribosome cluster of Leucochloridium paradoxum Carus, 1835 and L. perturbatum Pojmanska, 1967 were obtained. rDNA was extracted from 40 isolates of Leucochloridium sp. and analyzed using specific primers. The intraspecific genetically identity of morphologically detected L. paradoxum and L. perturbatum sporocysts was proven. A noticeable interspecific divergence between L. paradoxum and L. perturbatum was indicated. Using rDNA genotyping a case of double infection of snail Succinea sp. with L. paradoxum and L. perturbatum sporocysts was detected.

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基于rDNA序列的异绿蛭和异绿蛭的鉴定。
获得了1835年和1967年两种植物的DNA核糖体簇的全核苷酸序列。从40株白藻中提取rDNA,用特异引物对其进行分析。形态学检测到的悖论乳杆菌和摄动乳杆菌孢子囊的种内遗传同一性得到了证实。自相矛盾乳杆菌和摄动乳杆菌之间存在明显的种间差异。采用rDNA基因分型方法,检测了1例异乳杆菌和摄动乳杆菌孢子囊双重感染钉螺琥珀菌的病例。
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