Ofelia Maniti, Liberty François-Moutal, Marie-France Lecompte, Christian Vial, Michel Lagarde, Michel Guichardant, Olivier Marcillat, Thierry Granjon
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引用次数: 8
Abstract
4-Hydroxy-2-nonenal (4-HNE) is a reactive aldehyde and a lipid peroxidation product formed in biological tissues under physiological and pathological conditions. Its concentration increases with oxidative stress and induces deleterious modifications of proteins and membranes. Mitochondrial and cytosolic isoforms of creatine kinase were previously shown to be affected by 4-HNE. In the present study, we analyzed the effect of 4-HNE on mitochondrial creatine kinase, an abundant protein from the mitochondrial intermembrane space with a key role in mitochondrial physiology. We show that this effect is double: 4-HNE induces a step-wise loss of creatine kinase activity together with a fast protein aggregation. Protein-membrane interaction is affected and amyloid-like networks formed on the biomimetic membrane. These fibrils may disturb mitochondrial organisation both at the membrane and in the inter membrane space.
期刊介绍:
Cessation.
Molecular Membrane Biology provides a forum for high quality research that serves to advance knowledge in molecular aspects of biological membrane structure and function. The journal welcomes submissions of original research papers and reviews in the following areas:
• Membrane receptors and signalling
• Membrane transporters, pores and channels
• Synthesis and structure of membrane proteins
• Membrane translocation and targeting
• Lipid organisation and asymmetry
• Model membranes
• Membrane trafficking
• Cytoskeletal and extracellular membrane interactions
• Cell adhesion and intercellular interactions
• Molecular dynamics and molecular modelling of membranes.
• Antimicrobial peptides.