[Assessment of the quality of laboratory diagnosis of intestinal parasitic diseases by the laboratories participating in the Federal System of External Quality Assessment of Clinical Laboratory Testing].

V N Malakhov, A S Dovgalev, S Iu Astanina, A P Serdiuk
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Abstract

In 2010-2013, the quality of microscopic detection of the causative agents ofparasitic diseases in the feces has been assessed by the specialists of the laboratories of the therapeutic-and-prophylactic institutions (TPIs) and Hygiene and Epidemiology Centers, Russian Inspectorate for the Protection of Consumer Rights and Human Welfare, which are participants of the Federal System of External Quality Assessment of Clinical Laboratory Testing. Thirty-two specimens containing 16 species of human helminths and 4 species of enteric protozoa in different combinations were examined. The findings suggest that the quality of microscopic detection of the causative agents of parasitic diseases is low in the laboratories of health care facilities and that the specialists of the laboratories of TPIs and Hygiene and Epidemiology Centers, Russian Inspectorate for the Protection of Consumer Rights and Human Welfare, do not not possess the knowledge and skills necessary to make a laboratory diagnosis of helminths and enteric protozoa. The average detection rates of helminths and protozoa were at a level of 64 and 36%, respectively. The correct results showed that the proportion of helminths and protozoa were 94.5 and 5.5%, respectively. According to the biological and epidemiological classification of helminths, there were higher detection rates for contact group parasites (Enterobius vermicularis and Hymenolepis nana) and geohelminths (Ascaris, Trichuris trichiura, and others). Biohelminths (Opisthorchis, tapeworms, and others) Were detectable slightly worse.

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[参与联邦临床实验室检测外部质量评价体系的实验室对肠道寄生虫病的实验室诊断质量评价]。
2010-2013年,治疗和预防机构(tpi)实验室和俄罗斯保护消费者权利和人类福利监察局卫生和流行病学中心的专家对粪便中寄生虫病原体的显微检测质量进行了评估,这些实验室是联邦临床实验室检测外部质量评估系统的参与者。共检测了32份标本,其中包括16种人类蠕虫和4种不同组合的肠道原生动物。调查结果表明,在卫生保健设施的实验室中,对寄生虫病病原体进行显微镜检测的质量很低,而且,在tpd实验室和俄罗斯保护消费者权利和人类福利监察局卫生和流行病学中心的实验室中,专家并不具备对寄生虫和肠道原生动物进行实验室诊断所必需的知识和技能。寄生虫和原虫的平均检出率分别为64%和36%。正确的结果表明,线虫和原生动物的比例分别为94.5%和5.5%。根据寄生虫的生物学和流行病学分类,接触群寄生虫(蛲虫和膜孔虫)和地虫(蛔虫、毛线虫等)的检出率较高。生物蠕虫(蛇胸虫、绦虫等)的检测结果稍差。
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