Regulation of neutrophil infiltration into peritoneal cavity by laparoscopic gastrectomy.

Hepato-gastroenterology Pub Date : 2015-03-01

Mami Yoshii, Hiroaki Tanaka, Masaichi Ohira, Kazuya Muguruma, Tomohiro Lee, Katsunobu Sakurai, Naoshi Kubo, Kiyoshi Maeda, Kosei Hirakawa

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Abstract

Background/aims: Laparoscopic surgery is a minimally invasive operation developed for treating gastrointestinal malignancies. We aimed to characterize the differences in the intra-abdominal environment following open and laparoscopic surgeries.

Methodology: We investigated data of 48 patients who underwent gastrectomy between 2010 and 2012. We analyzed the mRNA expression of chemokines, indoleamine 2, 3-dioxygenase (IDO), and so on in peritoneal lavage fluid with real-time RT-PCR. We also determined the leukocyte population and calculated the granulocyte/lymphocyte (G/L) ratio in peritoneal lavage fluid using flow cytometry.

Results: CCL3 mRNA was significantly upregulated, whereas IDO mRNA was significantly downregulated, in the open group compared to the laparoscopic surgery group. Flow cytometry revealed that the G/L ratio was significantly higher in the open group.

Conclusions: We suggest that the production of chemokines and neutrophil infiltration into the abdominal cavity may be suppressed in the laparoscopic surgery. Thus, laparoscopic surgery may be beneficial in preserving local immunity.

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腹腔镜胃切除术对中性粒细胞腹腔浸润的调节。

背景/目的:腹腔镜手术是一种用于治疗胃肠道恶性肿瘤的微创手术。我们的目的是描述在开放和腹腔镜手术后腹内环境的差异。方法:我们调查了2010年至2012年间接受胃切除术的48例患者的资料。采用实时RT-PCR方法分析腹腔灌洗液中趋化因子、吲哚胺2,3 -双加氧酶(IDO)等mRNA的表达。我们还用流式细胞术测定了腹膜灌洗液中的白细胞群，并计算了粒细胞/淋巴细胞(G/L)的比值。结果:与腹腔镜手术组相比，开放组CCL3 mRNA显著上调，IDO mRNA显著下调。流式细胞术显示，开放组的G/L比值明显升高。结论:腹腔镜手术可能抑制了趋化因子的产生和中性粒细胞的腹腔浸润。因此，腹腔镜手术可能有利于保持局部免疫。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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