Jiwon Jung, Ji Yeun Kim, Ji-Soo Kwon, Sung-Cheol Yun, Sung-Han Kim
{"title":"Comparison of Waning Immunity Between Booster Vaccination and 2-Dose Vaccination With BNT162b2.","authors":"Jiwon Jung, Ji Yeun Kim, Ji-Soo Kwon, Sung-Cheol Yun, Sung-Han Kim","doi":"10.4110/in.2022.22.e31","DOIUrl":null,"url":null,"abstract":"S1 specific IgG Ab was measured using an in-house developed ELISA, and the data are presented in IU/ml. The interferon-gamma ELISPOT assay was used to assess the SARS-CoV-2-specific T cell response in isolated PBMCs. T cells were stimulated using SARS-CoV-2 spike-overlapping peptides (Miltenyi Biotec, Bergisch Gladbach, Germany), and the number of spot-forming cells per 5.0×10 5 PBMCs were counted with an automated ELISPOT reader (AID iSPOT; Autoimmun Diagnostika GmbH, Strassberg, Germany). A linear mixed regression model was used to compare the slope from the peak Ab titer to the lowest Ab titer (23 wk after the second vaccination) and the slope from the peak Ab titer","PeriodicalId":13307,"journal":{"name":"Immune Network","volume":null,"pages":null},"PeriodicalIF":4.3000,"publicationDate":"2022-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/06/bc/in-22-e31.PMC9433190.pdf","citationCount":"2","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Immune Network","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.4110/in.2022.22.e31","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2022/8/1 0:00:00","PubModel":"eCollection","JCR":"Q2","JCRName":"IMMUNOLOGY","Score":null,"Total":0}
引用次数: 2
Abstract
S1 specific IgG Ab was measured using an in-house developed ELISA, and the data are presented in IU/ml. The interferon-gamma ELISPOT assay was used to assess the SARS-CoV-2-specific T cell response in isolated PBMCs. T cells were stimulated using SARS-CoV-2 spike-overlapping peptides (Miltenyi Biotec, Bergisch Gladbach, Germany), and the number of spot-forming cells per 5.0×10 5 PBMCs were counted with an automated ELISPOT reader (AID iSPOT; Autoimmun Diagnostika GmbH, Strassberg, Germany). A linear mixed regression model was used to compare the slope from the peak Ab titer to the lowest Ab titer (23 wk after the second vaccination) and the slope from the peak Ab titer
期刊介绍：
Immune Network publishes novel findings in basic and clinical immunology and aims to provide a medium through which researchers in various fields of immunology can share and connect. The journal focuses on advances and insights into the regulation of the immune system and the immunological mechanisms of various diseases. Research that provides integrated insights into translational immunology is given preference for publication. All submissions are evaluated based on originality, quality, clarity, and brevity
京公网安备 11010802042870号
京ICP备2023020795号-1
分享
求助内容：
应助结果提醒方式：
扫码关注我们
