An additional medium renewal of D4 embryo culture improves the concordance of noninvasive chromosome screening with trophectoderm biopsy.

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY Accounts of Chemical Research Pub Date : 2022-12-01 Epub Date: 2022-09-22 DOI:10.1017/S0967199422000363

Hong-Xing Li, Yan Pang, Xue-Hong Zhang, Di Cao, Xiao-Ling Ma

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Abstract

Our research question was to evaluate the chromosome concordance of trophectoderm (TE) biopsy with noninvasive chromosome screening (NICS) using embryo culture medium renewed twice on Day 3 (D3) and Day 4 (D4). In this study, we evaluated 64 cycles with 223 biopsied blastocysts. These were categorized into two groups based on replacing embryo culture medium on D3 (control group) or on D3 and D4 (experimental group). The fundamental characteristics and main outcomes were compared. The concordance rates of NICS results with TE biopsy were determined according to next generation sequencing results. In total, 103 experimental and 120 control embryo cultures were collected, and the euploid status was analyzed using NICS technology. The overall concordance rates with TE biopsy of the experimental and control groups were 0.86 and 0.75, respectively. Statistically significant difference was found between the two groups. An additional medium renewal of the D4 embryo culture can improve the concordance of NICS with TE biopsy.

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D4胚胎培养的额外培养基更新提高了无创染色体筛查与滋养外胚层活检的一致性。

我们的研究问题是利用在第3天(D3)和第4天(D4)更新两次的胚胎培养基，用无创染色体筛查(NICS)评估滋养外胚层(TE)活检的染色体一致性。在这项研究中，我们评估了223个活检囊胚的64个周期。按在D3上更换胚培养基(对照组)和在D3和D4上更换胚培养基(试验组)分为两组。比较两组的基本特征和主要结局。根据下一代测序结果确定NICS结果与TE活检的一致性率。共收集103个实验胚和120个对照胚，利用NICS技术分析整倍体状态。实验组和对照组与TE活检的总体符合率分别为0.86和0.75。两组间差异有统计学意义。D4胚胎培养的额外培养基更新可以提高NICS与TE活检的一致性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.

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