β-Mercaptoethanol in culture medium improves cryotolerance of in vitro-produced bovine embryos.

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY Accounts of Chemical Research Pub Date : 2022-12-01 Epub Date: 2022-09-23 DOI:10.1017/S0967199422000338

Karine de Mattos, Camilo Andrés Pena-Bello, Karine Campagnolo, Gabriella Borba de Oliveira, Elvis Ticiani, César Augusto Pinzón-Osorio, Ana Laura da Silva Feijó, Higor da Silva Ferreira, José Luiz Rodrigues, Marcelo Bertolini, Alceu Mezzallira, Eduardo de Souza Ribeiro

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引用次数: 2

Abstract

The objective of this study was to investigate the effects of adding β-mercaptoethanol (βME) to culture medium of bovine in vitro-produced (IVP) embryos prior to or after vitrification on embryo development and cryotolerance. In Experiment I, Day-7 IVP blastocysts were vitrified and, after warming, cultured in medium containing 0, 50 or 100 μM βME for 72 h. Embryos cultured in 100 μM βME attained higher hatching rates (66.7%) than those culture in 0 (47.7%) and 50 (52.4%) μM βME. In Experiment II, IVP embryos were in vitro-cultured (IVC) to the blastocyst stage in 0 (control) or 100 μM βME, followed by vitrification. After warming, embryos were cultured for 72 h (post-warming culture, PWC) in 0 (control) or 100 μM βME, in a 2 × 2 factorial design: (i) CTRL-CTRL, control IVC and control PWC; (ii) CTRL-βME, control IVC and βME-supplemented PWC; (iii) βME-CTRL, βME-supplemented IVC and control PWC; or (iv) βME-βME, βME-supplemented IVC and βME-supplemented PWC. βME during IVC reduced embryo development (28.0% vs. 43.8%) but, following vitrification, higher re-expansion rates were seen in βME-CTRL (84.0%) and βME-βME (87.5%) than in CTRL-CTRL (71.0%) and CTRL-βME (73.1%). Hatching rates were higher in CTRL-βME (58.1%) and βME-βME (63.8%) than in CTRL-CTRL (36.6%) and βME-CTRL (42.0%). Total cell number in hatched blastocysts was higher in βME-βME (181.2 ± 7.4 cells) than CTRL-CTRL (139.0 ± 9.9 cells). Adding βME to the IVC medium reduced development but increased cryotolerance, whereas adding βME to the PWC medium improved embryo survival, hatching rates, and total cell numbers.

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在培养基中添加β-巯基乙醇可提高体外培养的牛胚胎的低温耐受性。

本研究旨在探讨在玻璃化前后的牛体外培养胚胎培养基中添加β-巯基乙醇(βME)对胚胎发育和低温耐受性的影响。实验1将第7天IVP囊胚玻璃化，加热后在含0、50或100 μM βME的培养基中培养72 h。100 μM βME中培养的囊胚的孵化率(66.7%)高于0(47.7%)和50 (52.4%)μM βME中培养的囊胚。实验二，在0(对照)或100 μM βME条件下体外培养IVP胚胎至囊胚期，然后玻璃化。升温后，胚胎在0(对照)或100 μM βME条件下培养72 h(升温后培养，PWC)，采用2 × 2因子设计:(i) CTRL-CTRL，控制IVC和PWC;(ii) CTRL-βME，对照IVC和β me补充PWC;(iii) βME-CTRL、β me -补充IVC和对照PWC;(iv) βME-βME、βME补充IVC和βME补充PWC。在IVC期间，βME降低了胚胎发育(28.0%比43.8%)，但玻璃化后，βME-CTRL和βME-βME的再膨胀率分别为84.0%和87.5%，高于CTRL-CTRL和CTRL-βME的再膨胀率(71.0%和73.1%)。CTRL-βME组和βME-βME组的孵化率分别为58.1%和63.8%，高于CTRL-CTRL组的36.6%和βME-CTRL组的42.0%。βME-βME的总细胞数(181.2±7.4个细胞)高于CTRL-CTRL(139.0±9.9个细胞)。在IVC培养基中添加βME降低了胚胎的发育，但增加了低温耐受性，而在PWC培养基中添加βME提高了胚胎存活率、孵化率和总细胞数。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.

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