Genome Sequence and Characterization of Acinetobacter Phage DMU1.

PHAGE (New Rochelle, N.Y.) Pub Date : 2021-03-01 Epub Date: 2021-03-17 DOI:10.1089/phage.2020.0043

Bailey M Pehde, Devon Niewohner, Faithe E Keomanivong, Michael D Carruthers

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Abstract

Background: There has been a recent resurgence of research on the characterization of Acinetobacter phage for therapeutic use due to the morbidity and mortality associated with treatment failures in cases of multidrug-resistant Acinetobacter baumannii infections. Materials and Methods: A bacteriophage isolated from activated sludge that targets A. baumannii ATCC19606 was characterized by electron microscopy, genome sequencing, comparative genomics, and a host range analysis. Results: The morphology of Acinetobacter phage DMU1 resembles phages in Siphoviridae. Comparative genomic and phylogenetic analyses reveal that DMU1 is a siphophage and is most closely related to Acinetobacter phage SH-Ab 15497. Out of the strains tested, DMU1 was found to only infect A. baumannii strains ATCC19606 and ATCC17978. Conclusion: Acinetobacter phage DMU1 belongs to the Siphoviridae family and is most closely related to Acinetobacter phage SH-Ab 15497. Small-scale host-range analysis of DMU1 indicates a host range that is likely limited to specific A. baumannii strains.

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不动杆菌噬菌体DMU1的基因组序列与特性研究。

背景:由于多药耐药鲍曼不动杆菌感染的发病率和死亡率与治疗失败相关，最近对治疗用途的不动杆菌噬菌体特征的研究重新兴起。材料和方法:从活性污泥中分离出一种靶向鲍曼不动杆菌ATCC19606的噬菌体，通过电子显微镜、基因组测序、比较基因组学和宿主范围分析对其进行了表征。结果:不动杆菌噬菌体DMU1的形态与Siphoviridae噬菌体相似。比较基因组学和系统发育分析表明，DMU1是一种噬菌体，与不动杆菌噬菌体SH-Ab 15497亲缘关系最密切。在测试的菌株中，发现DMU1仅感染鲍曼不动杆菌菌株ATCC19606和ATCC17978。结论:不动杆菌噬菌体DMU1属于siphoviriae科，与不动杆菌噬菌体SH-Ab 15497亲缘关系最密切。DMU1的小规模宿主范围分析表明，宿主范围可能仅限于特定的鲍曼不动杆菌菌株。

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PHAGE (New Rochelle, N.Y.)

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