Investigation of the Gastroprotective Effect of Betaine-Homocysteine Homeostasis on Oxidative Stress, Inflammation and Apoptosis in Ethanol-Induced Ulcer Model.

IF 2.1 4区 医学 Q2 SURGERY Journal of Investigative Surgery Pub Date : 2022-11-01 Epub Date: 2022-09-25 DOI:10.1080/08941939.2022.2126566
Ayşe Çakır Gündoğdu, Fatih Kar, Cansu Özbayer
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引用次数: 2

Abstract

Background: There is a growing interest in the use of natural compounds for the treatment of gastric ulcers. The multifunctional roles of betaine in various diseases make this natural substance a favorable pre-drug for ulcer treatment. This study aims to determine the competence of betaine in gastroprotection against ethanol-induced damage and to explore underlying mechanisms considering its effects on liver and kidney activity and blood parameters.Methods: Wistar albino rats were orally treated with vehicle (distilled water) or betaine (250 mg/kg) for twenty-one days and then ulcer formation was induced by ingestion of 75% ethanol. Gastric mucosal damage was evaluated by gross examination and histopathological analysis. Homocysteine levels, lipid peroxidation, total antioxidant status (TAS), total oxidant status (TAS), antioxidant enzymes and pro-inflammatory and anti-inflammatory cytokines levels were assessed by enzyme-linked immunosorbent assay (ELISA) or immunohistochemistry. Furthermore, routine biochemical tests were performed and hematological parameters were analyzed.Results: Betaine ameliorated any gastric mucosal damage and reduced homocysteine levels significantly. The TOS and malondialdehyde (MDA) levels were decreased while the TAS, glutathione (GSH) levels and catalase (CAT) activity were increased upon the betaine treatment. Betaine reduced apoptosis by regulating Bax and Bcl-2 levels, however, it did not alter inflammatory mediators. Additionally, betaine improved serum potassium (K+) and blood urea nitrogen (BUN) levels, whereas it increased alanine aminotransferase (ALT) levels and impaired hematological parameters.Conclusions: Altogether, these data illustrated that betaine exhibits a gastroprotective effect against ulcers through the homocysteine pathway by modulating oxidative stress in the gastric tissue; however, its systemic effects should not be ignored.

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甜菜碱-同型半胱氨酸稳态对乙醇性溃疡模型氧化应激、炎症和细胞凋亡保护作用的研究。
背景:人们对使用天然化合物治疗胃溃疡越来越感兴趣。甜菜碱在多种疾病中的多功能作用使这种天然物质成为治疗溃疡的良好药物。本研究旨在探讨甜菜碱对肝脏、肾脏活性和血液参数的影响,以确定甜菜碱对胃抗乙醇损伤的保护能力,并探讨其潜在的机制。方法:用培养液(蒸馏水)或甜菜碱(250 mg/kg)灌胃治疗Wistar白化大鼠21 d,然后灌胃75%乙醇诱导溃疡形成。通过大体检查和组织病理学分析评估胃粘膜损伤。通过酶联免疫吸附试验(ELISA)或免疫组织化学评估同型半胱氨酸水平、脂质过氧化、总抗氧化状态(TAS)、总氧化状态(TAS)、抗氧化酶以及促炎和抗炎细胞因子水平。常规生化检查及血液学指标分析。结果:甜菜碱可改善胃粘膜损伤,显著降低同型半胱氨酸水平。甜菜碱处理使TOS和丙二醛(MDA)水平降低,TAS、谷胱甘肽(GSH)水平和过氧化氢酶(CAT)活性升高。甜菜碱通过调节Bax和Bcl-2水平减少细胞凋亡,但不改变炎症介质。此外,甜菜碱提高了血清钾(K+)和血尿素氮(BUN)水平,增加了谷丙转氨酶(ALT)水平,损害了血液学参数。结论:总的来说,这些数据表明甜菜碱通过调节胃组织中的氧化应激,通过同型半胱氨酸途径对溃疡表现出胃保护作用;然而,其系统性影响不容忽视。
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来源期刊
CiteScore
4.20
自引率
0.00%
发文量
114
审稿时长
6-12 weeks
期刊介绍: Journal of Investigative Surgery publishes peer-reviewed scientific articles for the advancement of surgery, to the ultimate benefit of patient care and rehabilitation. It is the only journal that encompasses the individual and collaborative efforts of scientists in human and veterinary medicine, dentistry, basic and applied sciences, engineering, and law and ethics. The journal is dedicated to the publication of outstanding articles of interest to the surgical research community.
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