Immunochemical studies of Toxocara canis proteases.

IF 0.8 4区 医学 Q4 PARASITOLOGY Tropical biomedicine Pub Date : 2022-09-01 DOI:10.47665/tb.39.3.002
I F Abou-El-Naga, S M F El-Nassery, I A Sharaf
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引用次数: 1

Abstract

Proteases of nematodes play a crucial role in larval molting and, in addition to their active role in egg hatching, proteases are also considered a crucial factor in tissue invasion and connective tissue remodeling. In Toxocara canis, proteases play important roles throughout the complex life cycle. They can degrade components of a model of extracellular matrix, basement membranes and different physiological substrates. In the present study, measurements of the proteolytic activity of the perivitelline fluid (PF) surrounding Toxocara canis embryos at different stages of development, the hatching fluid (HF) surrounding the infective larvae, as well as the excretory secretory (ES) products of the larvae in the culture media were performed. Measurements were made using casein as substrate following the Sigma non-specific protease activity assay. The results showed that enzyme activity increased as the embryo matured. The infective larvae were found to continuously produce proteases in the surrounding HF and ES products after in vitro cultivation indicating that Toxocara canis proteases might be important for the worm in the egg and the host. Optimal enzymatic activity was found at pH 8. Incubation of the antiserum from infected mice with the HF and ES products decreased their proteolytic activities, suggesting that there may be a link between the proteases present in these fluids and the immune response.

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犬弓形虫蛋白酶的免疫化学研究。
线虫的蛋白酶在幼虫蜕皮过程中起着至关重要的作用,除了在卵孵化过程中发挥积极作用外,蛋白酶还被认为是组织侵袭和结缔组织重塑的关键因素。在犬弓形虫中,蛋白酶在复杂的生命周期中起着重要的作用。它们可以降解细胞外基质、基底膜和不同生理基质模型的成分。在本研究中,测量了不同发育阶段犬弓形虫胚胎周围卵泡液(PF)、感染幼虫周围的孵化液(HF)以及培养液中幼虫的排泄分泌(ES)产物的蛋白水解活性。在Sigma非特异性蛋白酶活性测定后,使用酪蛋白作为底物进行测量。结果表明,随着胚胎的成熟,酶活性逐渐增强。体外培养后发现,受感染的幼虫在周围HF和ES产物中持续产生蛋白酶,这表明犬弓形虫的蛋白酶可能对卵和宿主中的蠕虫很重要。pH为8时酶活性最佳。感染小鼠的抗血清与HF和ES产物的孵育降低了它们的蛋白水解活性,这表明这些液体中存在的蛋白酶与免疫反应之间可能存在联系。
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来源期刊
Tropical biomedicine
Tropical biomedicine 医学-寄生虫学
CiteScore
1.60
自引率
0.00%
发文量
63
审稿时长
6-12 weeks
期刊介绍: The Society publishes the Journal – Tropical Biomedicine, 4 issues yearly. It was first started in 1984. The journal is now abstracted / indexed by Medline, ISI Thompson, CAB International, Zoological Abstracts, SCOPUS. It is available free on the MSPTM website. Members may submit articles on Parasitology, Tropical Medicine and other related subjects for publication in the journal subject to scrutiny by referees. There is a charge of US$200 per manuscript. However, charges will be waived if the first author or corresponding author are members of MSPTM of at least three (3) years'' standing.
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