Effect of ozone versus naringin on testicular injury in experimentally induced ulcerative colitis in adult male albino rats.

IF 1.1 4区 医学 Q4 MICROSCOPY Ultrastructural Pathology Pub Date : 2022-09-03 DOI:10.1080/01913123.2022.2132337
Abeer M Azmy, Bassant T Abd Elbaki, Mohammed A Ali, Abeer A Mahmoud
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引用次数: 1

Abstract

Testicular dysfunction is caused by the continuous inflammation and oxidative stress that are present at the local site in ulcerative colitis (UC) spreading to the testes via systemic circulation. The influence of ozone and naringine on colitis-mediated testicular dysfunction was investigated in this study. Forty-eight adult male rats were divided into four groups: I control group, II dextran sodium sulfate (DSS) UC-induced group, III DSS+naringine, and IV DSS+ozone groups. UC was induced in groups II, III, and IV using 0.1 ml of 4% DSS in their drinking water per day for 6 days by gastric gavage. All animals were sacrificed 45 days from the start. Blood samples were obtained to estimate serum testosterone hormone. Testicular tissues were processed for measurement of tissue malondialdehyde (MDA) and examined by light and electron microscopes. Ultrastructurally, group II revealed a relatively thick basement membrane enveloping the seminiferous tubule. Sertoli cell cytoplasm appears rarified with wide intracellular spaces, vacuoles, and multiple lysosomes; distorted spermatogonia with electron dense nuclei and cytoplasm; and primary spermatocytes with small nuclei and electron dense cytoplasm. Abnormal sperm profiles were visible in middle pieces, mid, principle, and end pieces that were markedly affected with disorganization of axoneme and outer dense fibers. Leydig cells revealed dilated cisternae of smooth endoplasmic reticulum. Morphometric and statistical analyses were performed. Group III showed some improvement; however, group IV showed more improvement. The results indicated that ozone caused marked improvement than naringine against UC-induced testicular damage via their antioxidant and anti-inflammatory properties.

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臭氧与柚皮苷对实验性溃疡性结肠炎成年雄性白化大鼠睾丸损伤的影响。
溃疡性结肠炎(UC)局部持续的炎症和氧化应激通过体循环扩散到睾丸,引起睾丸功能障碍。本研究探讨臭氧和柚皮碱对结肠炎所致睾丸功能障碍的影响。将48只成年雄性大鼠分为4组:ⅰ对照组、ⅱ葡聚糖硫酸钠(DSS) uc诱导组、ⅲDSS+柚皮苷组、ⅳDSS+臭氧组。II、III、IV组小鼠每天在饮水中加入0.1 ml 4% DSS,通过胃灌胃诱导UC,连续6 d。所有动物从开始的第45天开始被处死。采集血样,测定血清睾酮水平。对睾丸组织进行处理,测定组织丙二醛(MDA),并进行光镜和电镜检查。在超微结构上,II组显示有相对较厚的基膜包裹着精管。支持细胞细胞质多样,胞内间隙宽,有液泡,溶酶体多;畸变精原细胞,细胞核和细胞质电子密集;原代精母细胞细胞核小,细胞质电子致密。精子中片、中片、主片和终片均可见异常精子,轴突和外层致密纤维组织紊乱。间质细胞显示平滑内质网池扩张。进行形态计量学和统计学分析。第三组有一定改善;然而,IV组表现出更大的改善。结果表明,臭氧对uc致睾丸损伤的抗氧化和抗炎作用明显优于柚皮碱。
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来源期刊
Ultrastructural Pathology
Ultrastructural Pathology 医学-病理学
CiteScore
2.00
自引率
10.00%
发文量
40
审稿时长
6-12 weeks
期刊介绍: Ultrastructural Pathology is the official journal of the Society for Ultrastructural Pathology. Published bimonthly, we are the only journal to be devoted entirely to diagnostic ultrastructural pathology. Ultrastructural Pathology is the ideal journal to publish high-quality research on the following topics: Advances in the uses of electron microscopic and immunohistochemical techniques Correlations of ultrastructural data with light microscopy, histochemistry, immunohistochemistry, biochemistry, cell and tissue culturing, and electron probe analysis Important new, investigative, clinical, and diagnostic EM methods.
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