Mesenchymal stem cells have ameliorative effect on the colitis model via Nrf2/HO-1 pathway.

IF 1.1 4区医学 Q3 SURGERY Acta cirurgica brasileira Pub Date : 2022-10-10 eCollection Date: 2022-01-01 DOI:10.1590/acb370704

Mehmet Fatih Bozkurt, Muhammed Nasir Bhaya, Cengiz Dibekoğlu, Ayberk Akat, Utku Ateş, Oytun Erbaş

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Abstract

Purpose: To evaluate the ameliorative effect of mesenchymal stem cells (MSCs) on acetic acid colitis model via Nrf2/HO-1 pathway in rats.

Methods: In this study, 30 rats were divided into three groups. Acute colitis was induced by rectal administration of 4% solution of acetic acid. MSCs were injected intraperitoneally in the treatment group.

Results: Increased levels of tumor necrosis factor-α (TNF-α), pentraxin-3, and malondialdehyde (MDA) in colitis group were revealed biochemically. Increased level of TNF-α and decreased levels of Nrf2 and interleukin-10 (IL-10) were observed in rectum tissues. Increased fibrous tissue proliferation, vascularization and inflammatory cell infiltration were described in the colitis group. Significant improvement was observed in MSCs treated group histopathologically. Increased immunopositivity of TNF-α, vascular endothelial growth factor (VEGF) and CD68 markers was observed in the colitis group cells, and decreased level of this positivity was observed in MSCs treated group.

Conclusions: Biochemical, histopathological and immunohistochemical results strongly support the ameliorative effect of MSCs against acetic induced colitis model via Nrf2/HO-1 pathway in rats.

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间充质干细胞通过Nrf2/HO-1通路对结肠炎模型有改善作用。

目的:探讨间充质干细胞(MSCs)通过Nrf2/HO-1通路对大鼠醋酸性结肠炎模型的改善作用。方法:将30只大鼠分为3组。用4%醋酸溶液直肠灌胃诱导急性结肠炎。治疗组采用腹腔注射MSCs。结果:结肠炎组肿瘤坏死因子-α (TNF-α)、戊曲霉素-3、丙二醛(MDA)水平明显升高。直肠组织中TNF-α水平升高，Nrf2和白细胞介素-10 (IL-10)水平降低。结肠炎组纤维组织增生、血管化和炎症细胞浸润增加。MSCs处理组在组织病理学上有明显改善。结肠炎组细胞中TNF-α、血管内皮生长因子(VEGF)和CD68标志物免疫阳性水平升高，MSCs处理组细胞免疫阳性水平降低。结论:生物化学、组织病理学和免疫组化结果有力支持MSCs通过Nrf2/HO-1途径对大鼠醋酸诱导结肠炎模型的改善作用。

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