HPV-related Oropharyngeal Squamous Cell Carcinoma: p16INK4A Immunohistochemistry or HPV Genotyping?

IF 1.6 4区医学 Q4 ONCOLOGY Anticancer research Pub Date : 2015-09-01

Maria Orsaria, Stefania Marzinotto, Luca De Marchi, Vittorio Giacomarra, Silvio Boria, Corrado Rubini, Ambrogio Pietro Londero, Carla Di Loreto, Laura Mariuzzi

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Abstract

Background: Infection with high-risk human papillomavirus (HPV) is linked to a sub-group of squamous cell oropharyngeal tumors (OPSCC). Our aim was to compare an HPV Polymerase Chain Reaction (PCR) assay and p16(INK4A) expression status by immunohistochemistry (IHC) as a surrogate marker.

Materials and methods: This was a retrospective study considering patients affected by squamous cell oropharyngeal tumors. All included samples were processed for IHC for p16(INK4A) and tested by PCR for detection of HPV DNA and HPV genotyping.

Results: A total of 84 patients affected by squamous cell oropharyngeal tumors were included and tested. A significant positive correlation was found between HPV PCR and p16(INK4A) IHC but the agreement was poor (k coefficient of 0.25). In fact, the sensitivity of p16(INK4A) IHC positivity in detecting HPV PCR positivity was low (28.21%, 95% confidence interval=16.54% - 43.78%).

Conclusion: Positivity of p16(INK4A) by IHC had a low sensitivity in detecting HPV DNA and our results suggest the need at least to test p16(INK4A) IHC- negative samples using HPV PCR to increase detection accuracy and provide valuable information for the clinical management of these patients.

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HPV相关口咽鳞状细胞癌:p16INK4A免疫组化还是HPV基因分型?

背景:感染高危人乳头瘤病毒(HPV)与鳞状细胞口咽肿瘤(OPSCC)亚群有关。我们的目的是比较HPV聚合酶链反应(PCR)测定和p16(INK4A)的表达状况，免疫组织化学(IHC)作为替代标志物。材料和方法:这是一项回顾性研究，考虑了鳞状细胞口咽肿瘤患者。所有纳入的样本均进行p16(INK4A)的免疫组化处理，并进行PCR检测HPV DNA和HPV基因分型。结果:共纳入84例口咽鳞状细胞瘤患者。HPV PCR与p16(INK4A) IHC呈显著正相关，但一致性较差(k系数为0.25)。事实上，p16(INK4A) IHC阳性检测HPV PCR阳性的敏感性较低(28.21%，95%可信区间=16.54% ~ 43.78%)。结论:IHC检测p16(INK4A)阳性对HPV DNA检测的敏感性较低，提示至少需要用HPV PCR检测p16(INK4A) IHC阴性的样本，以提高检测的准确性，为这类患者的临床管理提供有价值的信息。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Anticancer research 医学-肿瘤学

CiteScore

3.70

自引率

10.00%

发文量

566

审稿时长

2 months

期刊介绍： ANTICANCER RESEARCH is an independent international peer-reviewed journal devoted to the rapid publication of high quality original articles and reviews on all aspects of experimental and clinical oncology. Prompt evaluation of all submitted articles in confidence and rapid publication within 1-2 months of acceptance are guaranteed. ANTICANCER RESEARCH was established in 1981 and is published monthly (bimonthly until the end of 2008). Each annual volume contains twelve issues and index. Each issue may be divided into three parts (A: Reviews, B: Experimental studies, and C: Clinical and Epidemiological studies). Special issues, presenting the proceedings of meetings or groups of papers on topics of significant progress, will also be included in each volume. There is no limitation to the number of pages per issue.