{"title":"Development and validation of a diagnostic test for Ridge allele copy number in Rhodesian Ridgeback dogs.","authors":"Jennifer Turner Waldo, Kasandra Santana Diaz","doi":"10.1186/s40575-015-0013-x","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>The breed-defining dorsal ridge in Rhodesian Ridgeback dogs is the result of a 133,000 base pair duplication on chromosome 18. Because this trait is dominant, heterozygous dogs cannot be discriminated from those with two copies of the Ridge allele.</p><p><strong>Results: </strong>A quantitative PCR test was developed and dogs of known genotype were used as test subjects. In all cases, the correct genotype was determined experimentally.</p><p><strong>Conclusions: </strong>This work provides a rapid and accurate methodology for determining dog genotype with respect to the Ridge allele.</p>","PeriodicalId":91060,"journal":{"name":"Canine genetics and epidemiology","volume":"2 ","pages":"2"},"PeriodicalIF":0.0000,"publicationDate":"2015-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s40575-015-0013-x","citationCount":"3","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Canine genetics and epidemiology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1186/s40575-015-0013-x","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2015/1/1 0:00:00","PubModel":"eCollection","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 3
Abstract
Background: The breed-defining dorsal ridge in Rhodesian Ridgeback dogs is the result of a 133,000 base pair duplication on chromosome 18. Because this trait is dominant, heterozygous dogs cannot be discriminated from those with two copies of the Ridge allele.
Results: A quantitative PCR test was developed and dogs of known genotype were used as test subjects. In all cases, the correct genotype was determined experimentally.
Conclusions: This work provides a rapid and accurate methodology for determining dog genotype with respect to the Ridge allele.
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