Detection of Apoptotic Cells in a Rabbit Model with Atherosclerosis-Like Lesions Using the Positron Emission Tomography Radiotracer [18F]ML-10.
IF 2.2 4区 医学Q3 BIOCHEMICAL RESEARCH METHODSMolecular ImagingPub Date : 2015-01-01
Fabien Hyafil, Alexy Tran-Dinh, Samuel Burg, Sébastien Leygnac, Liliane Louedec, Milan Milliner, Rana Ben Azzouna, Ayelet Reshef, Miri Ben Ami, Olivier Meilhac, Dominique Le Guludec
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引用次数: 0
Abstract
[18F]ML-10 (2-(5-fluoro-pentyl)-2-methylmalonic acid) is a positron emission tomography (PET) radiotracer that accumulates in cells presenting apoptosis-specific membrane alterations. The aim of this study was to test whether [18F]ML-10 allows for the detection of apoptotic cells located in atherosclerotic plaques in rabbits. Atherosclerotic plaques were induced in the aortas of five rabbits, and five additional rabbits were used as controls. Activity in the aortas was quantified in vivo and ex vivo. The localization of [18F]ML-10 to the aortic wall was identified by autoradiography. Average target to background ratios measured in vivo by PET were higher in the aortas of atherosclerotic rabbits compared with those of control rabbits (2.00 ± 0.52 vs 1.22 ± 0.30; p < .05). Differences in [18F]ML-10 uptake between atherosclerotic and control aortas were confirmed ex vivo by PET and gamma counting (23.9 ± 11.2 vs 1.1 ± 2.4 counts/pixel; p <.05; 3.6 ± 2.0 vs 0.05 ± 0.05 % of injected activity/g; p < .05, respectively). Strong correlation was observed between the accumulation of [18F]ML-10 in aortic segments as detected by autoradiography and the number of apoptotic cells on corresponding histologic sections (r2 = .75; p < .05). In this study, we found that atherosclerotic plaques rich in apoptotic cells can be detected with [18F]ML-10 and PET.
[18F]ML-10(2-(5-氟戊基)-2-甲基丙二酸)是一种正电子发射断层扫描(PET)放射性示踪剂,在呈现凋亡特异性膜改变的细胞中积累。本研究的目的是测试[18F]ML-10是否允许检测位于家兔动脉粥样硬化斑块中的凋亡细胞。在5只家兔的主动脉中诱导动脉粥样硬化斑块,另外5只家兔作为对照。在体内和离体对主动脉活性进行量化。[18F]ML-10在主动脉壁的定位通过放射自显像确定。PET在体内测得的动脉粥样硬化兔主动脉平均靶本比高于对照组(2.00±0.52 vs 1.22±0.30;P < 0.05)。通过PET和伽马计数(23.9±11.2 vs 1.1±2.4计数/像素)证实动脉粥样硬化和对照主动脉[18F]ML-10摄取的体外差异;p
Molecular ImagingBiochemistry, Genetics and Molecular Biology-Biotechnology
自引率
3.60%
发文量
21
期刊介绍:
Molecular Imaging is a peer-reviewed, open access journal highlighting the breadth of molecular imaging research from basic science to preclinical studies to human applications. This serves both the scientific and clinical communities by disseminating novel results and concepts relevant to the biological study of normal and disease processes in both basic and translational studies ranging from mice to humans.