Effects of Temperature and pH on the Activities of Catechol 2,3-dioxygenase Obtained from Crude Oil Contaminated Soil in Ilaje, Ondo State, Nigeria.

Q3 Immunology and Microbiology Open Microbiology Journal Pub Date : 2015-07-31 eCollection Date: 2015-01-01 DOI:10.2174/1874285801509010084

O F Olukunle, O Babajide, B Boboye

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引用次数: 16

Abstract

Enrichment technique was employed for the isolation of the crude oil degrading bacteria. The isolated bacteria were screened for their degradative ability and the best degrading bacteria were selected based on their growth. Specific activities of Catechol-2,3-dioxygenase and effects of temperature and pH and their stabilities on the enzyme relative activities were observed. Bacteria isolated from the soil sample include; Bacillus cereus, B. amyloliquficiens, B. firmus, Acinetobacter calcoaceticus, Pseudomonas sp. P. fluorescens, P.putida, P.aeruginosa, Achromobacter xylosoxidans and Achromobacter sp. Screening of the degradative ability of the bacteria revealed P. aeruginosa, Bacillus cereus, Acinetobacter calcoaceticus and Achromobacter sp. to be the best degraders. The pH and temperature range with time for the enzyme activity were 6.0-8.0 and 30(o)C-50(o)C respectively. The enzyme exhibited activity that was slightly more tolerant to alkaline pH. Therefore, engineering of Catechol 2,3-dioxygenase may be employed for application on bioremediation of polluted sites.

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温度和pH对尼日利亚Ondo州Ilaje原油污染土壤中邻苯二酚2,3-双加氧酶活性的影响

采用富集技术对原油降解菌进行分离。对分离菌的降解能力进行筛选，并根据其生长情况筛选出最佳降解菌。观察了儿茶酚-2,3-双加氧酶的比活性、温度和pH及其稳定性对酶相对活性的影响。从土壤样品中分离出的细菌包括;蜡样芽孢杆菌、解淀粉芽孢杆菌、硬芽孢杆菌、钙酸不动杆菌、荧光假单胞菌、恶臭假单胞菌、铜绿假单胞菌、氧化木色不动杆菌和无色杆菌等细菌的降解能力筛选表明，铜绿假单胞菌、蜡样芽孢杆菌、钙酸不动杆菌和无色杆菌是降解能力最好的细菌。酶活性的pH和温度随时间变化范围分别为6.0 ~ 8.0和30(0)~ 50(0)C。该酶对碱性ph值的耐受性稍强。因此，邻苯二酚2,3-双加氧酶工程可用于污染场地的生物修复。

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来源期刊

Open Microbiology Journal Immunology and Microbiology-Immunology and Microbiology (all)

CiteScore

1.80

自引率

0.00%

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期刊介绍： The Open Microbiology Journal is a peer-reviewed open access journal which publishes research articles, reviews/mini-reviews, case studies, guest edited thematic issues and short communications/letters covering theoretical and practical aspects of Microbial systematics, evolutionary microbiology, immunology, virology, parasitology , bacteriology, mycology, phycology, protozoology, microbial ecology, molecular biology, microbial physiology, biochemistry, microbial pathogenesis, host-microbe interaction, systems microbiology, synthetic microbiology, bioinformatics. The Open Microbiology Journal , a peer-reviewed journal, is an important and reliable source of current information on developments in the field. The emphasis will be on publishing quality papers rapidly and freely available to researchers worldwide.