The Immunogenicity of Peptoid-Protein Conjugates.

Journal of vaccines & vaccination Pub Date : 2016-08-01 Epub Date: 2016-07-11 DOI:10.4172/2157-7560.1000329
Allison Case, Angela Desmond, Daniel Lopes, Kelly Dye, Kelly Mapes, Stephen Ruback, Iliodora Pop, Jiyeun Kate Kim, Pavitra Chakravarty, Joan E Smallshaw, Laurentiu M Pop, Ellen S Vitetta
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引用次数: 2

Abstract

We demonstrate that a peptoid composed of five monomers and attached via a maleimide linker to a carrier protein elicits anti-peptoid, anti-linker and anti-carrier antibodies in rabbits. Specific anti-peptoid antibodies were affinity purified and used to reproducibly retrieve three specific peptoid-coupled beads from 20,000 irrelevant peptoid-beads using magnetic screening.

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肽蛋白偶联物的免疫原性。
我们证明了一种由5个单体组成的类肽,通过马来酰亚胺连接剂连接到载体蛋白上,在兔子体内引发抗类肽、抗连接剂和抗载体抗体。特异性抗肽类抗体被亲和纯化,并使用磁性筛选从20,000个不相关的肽类微球中可重复地检索三个特异性肽类偶联微球。
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