Allison Case, Angela Desmond, Daniel Lopes, Kelly Dye, Kelly Mapes, Stephen Ruback, Iliodora Pop, Jiyeun Kate Kim, Pavitra Chakravarty, Joan E Smallshaw, Laurentiu M Pop, Ellen S Vitetta
{"title":"The Immunogenicity of Peptoid-Protein Conjugates.","authors":"Allison Case, Angela Desmond, Daniel Lopes, Kelly Dye, Kelly Mapes, Stephen Ruback, Iliodora Pop, Jiyeun Kate Kim, Pavitra Chakravarty, Joan E Smallshaw, Laurentiu M Pop, Ellen S Vitetta","doi":"10.4172/2157-7560.1000329","DOIUrl":null,"url":null,"abstract":"<p><p>We demonstrate that a peptoid composed of five monomers and attached <i>via</i> a maleimide linker to a carrier protein elicits anti-peptoid, anti-linker and anti-carrier antibodies in rabbits. Specific anti-peptoid antibodies were affinity purified and used to reproducibly retrieve three specific peptoid-coupled beads from 20,000 irrelevant peptoid-beads using magnetic screening.</p>","PeriodicalId":74006,"journal":{"name":"Journal of vaccines & vaccination","volume":"7 4","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2016-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4172/2157-7560.1000329","citationCount":"2","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of vaccines & vaccination","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.4172/2157-7560.1000329","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2016/7/11 0:00:00","PubModel":"Epub","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 2
Abstract
We demonstrate that a peptoid composed of five monomers and attached via a maleimide linker to a carrier protein elicits anti-peptoid, anti-linker and anti-carrier antibodies in rabbits. Specific anti-peptoid antibodies were affinity purified and used to reproducibly retrieve three specific peptoid-coupled beads from 20,000 irrelevant peptoid-beads using magnetic screening.