Cannabinoid receptor signaling induces proliferation but not neurogenesis in the mouse olfactory epithelium.

Neurogenesis (Austin, Tex.) Pub Date : 2016-01-13 eCollection Date: 2016-01-01 DOI:10.1080/23262133.2015.1118177
Chelsea R Hutch, Colleen C Hegg
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引用次数: 12

Abstract

The olfactory epithelium actively generates neurons through adulthood, and this neurogenesis is tightly regulated by multiple factors that are not fully defined. Here, we examined the role of cannabinoids in the regulation of neurogenesis in the mouse olfactory epithelium. In vivo proliferation and cell lineage studies were performed in mice (C57BL/6 and cannabinoid type 1 and 2 receptor deficient strains) treated with cannabinoids directly (WIN 55,212-2 or 2-arachidonylglycerol ether) or indirectly via inhibition of cannabinoid hydrolytic enzymes. Cannabinoids increased proliferation in neonatal and adult mice, and had no effect on proliferation in cannabinoid type 1 and 2 receptor deficient adult mice. Pretreatment with the cannabinoid type1 receptor antagonist AM251 decreased cannabinoid-induced proliferation in adult mice. Despite a cannabinoid-induced increase in proliferation, there was no change in newly generated neurons or non-neuronal cells 16 d post-treatment. However, cannabinoid administration increased apoptotic cell death at 72 hours post-treatment and by 16 d the level of apoptosis dropped to control levels. Thus, cannabinoids induce proliferation, but do not induce neurogenesis nor non-neuronal cell generation. Cannabinoid receptor signaling may regulate the balance of progenitor cell survival and proliferation in adult mouse olfactory epithelium.

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大麻素受体信号传导诱导小鼠嗅上皮细胞增殖,但不诱导神经发生。
嗅觉上皮在成年期积极产生神经元,这种神经发生受到多种因素的严格调节,这些因素尚未完全确定。在这里,我们研究了大麻素在调节小鼠嗅上皮神经发生中的作用。在小鼠(C57BL/6和大麻素1型和2型受体缺陷菌株)中,通过大麻素直接(WIN 55,212-2或2-花生四烯基甘油醚)或通过抑制大麻素水解酶间接处理,进行了体内增殖和细胞谱系研究。大麻素增加了新生小鼠和成年小鼠的增殖,而对大麻素1型和2型受体缺乏的成年小鼠的增殖没有影响。大麻素1型受体拮抗剂AM251预处理可降低大麻素诱导的成年小鼠增殖。尽管大麻素诱导增殖增加,但在处理16天后,新生成的神经元或非神经元细胞没有变化。然而,给药后72小时,大麻素增加了凋亡细胞的死亡,到16 d,凋亡水平下降到对照水平。因此,大麻素诱导增殖,但不诱导神经发生或非神经元细胞的产生。大麻素受体信号可能调节成年小鼠嗅上皮祖细胞存活和增殖的平衡。
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