[CYTOSKELETON ORIENTATION IN THE EPIDERMAL CELLS OF ROOTS FORMED DE NOVO ON LEAF EXPLANTS UNDER CLINOROTATION].

TSitologiia i genetika Pub Date : 2016-03-01
I V Bulavin
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Abstract

Root anatomy, cytoskeleton orientation and cell wall thickness in cells of the roots formed de novo in vitro under clinorotation (simulated microgravity) were investigated. Structure of the embryonic roots and of the roots formed de novo in cambium cells of the leaf petiole explants was shown to be similar. Root cell differentiation in vitro under clinorotation did not differ from that in control. Changes of tubulin microtubules' orientation in the epidermis of the distal elongation zone were observed under clinorotation that seems to be associated with specific physiological properties of the cells. Under clinorotation, the tendency of cell wall thinning was detected in the root cells formed in vitro.

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[叶片外植体在植物化作用下重新形成的根表皮细胞的细胞骨架取向]。
研究了在模拟微重力条件下体外新生根的解剖结构、细胞骨架取向和细胞壁厚度。胚根与叶柄外植体形成层细胞新生根的结构相似。根细胞在离体旋转下的分化与对照无明显差异。在倾斜旋转下,观察到远端延伸区表皮的微管蛋白微管取向的变化,这似乎与细胞的特定生理特性有关。体外培养的根细胞在旋光作用下有细胞壁变薄的趋势。
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