Intraspecies comparative genomics of three strains of Orientia tsutsugamushi with different antibiotic sensitivity

Genomics Data Pub Date : 2017-06-01 DOI:10.1016/j.gdata.2017.03.012

Hsiao-Mei Liao , Chien-Chung Chao , Haiyan Lei , Bingjie Li , Shien Tsai , Guo-Chiuan Hung , Wei-Mei Ching , Shyh-Ching Lo

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引用次数: 7

Abstract

We recently reported the genome of Orientia tsutsugamushi (OT) strain Karp (GenBank Accession #: NZ_LYMA00000000.2, https://www.ncbi.nlm.nih.gov/nuccore/NZ_LYMA00000000.2) with > 2 Mb in size through clone-based sequencing and high throughput genomic shotgun sequencing (HTS). The genomes of OT strains AFSC4 and AFSC7 were similarly sequenced by HTS Since strains AFSC4 (GenBank Accession #: NZ_LYMT00000000.1, https://www.ncbi.nlm.nih.gov/nuccore/1035784408) and AFSC7 (GenBank Accession #: NZ_LYMB00000000.1, https://www.ncbi.nlm.nih.gov/nuccore/1035854767) were more resistant to antibiotics than strain Karp, we conducted comparative analysis of the three draft genomes annotated by RAST server aimed to identify possible genetic bases of difference in microbial antibiotic sensitivity. Intraspecies comparative genomics analysis of the three OT strains revealed that two ORFs encoding hypothetical proteins in both strains AFSC4 and AFSC7 are absent in strain Karp.

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不同抗生素敏感性恙虫东方体的种内比较基因组学研究

我们最近报道了恙虫病东方体(OT)菌株Karp (GenBank Accession #: nz_lyma000000002, https://www.ncbi.nlm.nih.gov/nuccore/NZ_LYMA00000000.2)的基因组与>通过基于克隆的测序和高通量基因组霰弹枪测序(HTS)获得2mb的大小。由于菌株AFSC4 (GenBank Accession #: nz_lymt000000001, https://www.ncbi.nlm.nih.gov/nuccore/1035784408)和AFSC7 (GenBank Accession #:nz_lymb000000001, https://www.ncbi.nlm.nih.gov/nuccore/1035854767)比菌株Karp对抗生素更耐药，我们通过RAST server注释的三个草图基因组进行比较分析，旨在寻找微生物抗生素敏感性差异的可能遗传基础。对3株OT菌株的种内比较基因组学分析显示，在菌株Karp中没有编码菌株AFSC4和AFSC7中假设蛋白的两个orf。

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Genomics Data

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12 weeks