EFFECTS OF DIFFERENT HYDROPONIC SUBSTRATE COMBINATIONS AND WATERING REGIMES ON PHYSIOLOGICAL AND ANTI-FUNGAL PROPERTIES OF SIPHONOCHILUS AETHIOPICUS.

African journal of traditional, complementary, and alternative medicines : AJTCAM Pub Date : 2017-03-01 eCollection Date: 2017-01-01 DOI:10.21010/ajtcam.v14i3.10

Xego S, Kambizi L, Nchu F

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引用次数: 2

Abstract

Background: Production of medicinal plants in controlled environments, particularly hydroponic technology, provides opportunities for high quality biomass accumulation and optimizes production of secondary metabolites. Applying special watering regimes in combination with efficient soil draining is an encouraging new tool for the production of pharmaceutical relevant plants. The purpose of this paper was to evaluate the effect of substrate combinations and watering regimes on nutrient uptake, anti-F. oxysporum activity and secondary metabolite profile of S. aethiopicus.

Materials and methods: Coir was used as the main component for the preparation of media in different combinations; TI (Coir + vermiculite + perlite + bark), T2 (Coir + bark), T3 (Coir + perlite) and T4 (Coir + vermiculite). Plants in different treatments were grown under two watering regimes: 3 and 5-days watering intervals. At 9 weeks post treatment, plants were harvested, oven dried and tissue nutrient content, anti-F. oxysporum activity and secondary metabolites were analyzed.

Results: The results showed that there were significant differences (P < 0.05) on the uptake of P, K, N, Mg, Fe, Cu, B and NH4^-.The highest mean values for most nutrients were obtained in treatments under 3-days interval. Acetone extracts of S. aethiopicus under 5-days interval were the most bioactive against F. oxysporum. The MIC values obtained are relatively lower for the rhizomes, ranging from 0.078 - 0.3125 mg/ml compared to the higher MIC values (0.375 - 0.75 mg/ml) obtained in the leaves. LC-MS analysis of acetone extracts revealed the presence of phytochemicals such as caffeic acid, quercetin, p-hydroxybenzoic acid, rutin, kaempferol, epicatechin, naringenin, hesperetin and protocatechuic acid.

Conclusion: The antimicrobial activity and/or the phytochemical profile of the crude extracts were affected by watering regimes.

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不同水培基质组合及浇水方式对埃塞俄比亚虹吸蝇生理及抗真菌特性的影响。

背景:在受控环境下生产药用植物，特别是水培技术，为高质量生物量积累提供了机会，并优化了次生代谢物的生产。应用特殊的浇水制度结合有效的土壤排水是一种令人鼓舞的新工具，用于制药相关植物的生产。研究了不同基质组合和不同浇水方式对土壤养分吸收、抗真菌活性和抗真菌活性的影响。埃塞俄比亚霉尖孢菌活性及次生代谢物谱。材料与方法:以椰胶为主要组分，制备不同组合的培养基;TI(椰油+蛭石+珍珠岩+树皮)、T2(椰油+树皮)、T3(椰油+珍珠岩)和T4(椰油+蛭石)。不同处理的植株在3天和5天的浇水间隔下生长。处理后9周，收获植株，烘箱干燥，组织养分含量、抗f。分析了尖孢菌活性和次生代谢产物。结果:试验结果表明，各品种对P、K、N、Mg、Fe、Cu、B、NH4-的吸收量差异有统计学意义(P < 0.05)。大多数营养物质的平均值在间隔3 d的处理中最高。间隔5 d的埃塞俄比亚葡萄球菌丙酮提取物对尖孢镰刀菌的生物活性最高。根茎的MIC值相对较低，为0.078 ~ 0.3125 mg/ml，而叶片的MIC值较高(0.375 ~ 0.75 mg/ml)。丙酮提取物的LC-MS分析显示存在咖啡酸、槲皮素、对羟基苯甲酸、芦丁、山奈酚、表儿茶素、柚皮素、橙皮素和原儿茶酸等植物化学物质。结论:不同浇水方式对粗提物的抑菌活性和/或植物化学特征有影响。

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African journal of traditional, complementary, and alternative medicines : AJTCAM

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