Cheng Kai, Zhang Denglan, Deng Jingxuan, Zhao Yijun
{"title":"Isolation and Characterization of an Aeromonas punctata Bacteriophage.","authors":"Cheng Kai, Zhang Denglan, Deng Jingxuan, Zhao Yijun","doi":"10.2174/1874120701509010185","DOIUrl":null,"url":null,"abstract":"<p><p>An <i>Aeromonas punctata</i> bacteriophage, named as DH1, was isolated from East Lake, Wuhan city, China. Morphologically, phage DH1 showed a typical <i>Myoviridae</i> structure consisting of an isometric head (50 nm in diameter) and a visible tail. The bacteriophage had a latent period of about 90 minutes and an average burst size of about 125 PFU•Cell<sup>-1</sup>. Restriction enzyme pattern of the bacteriophage's genome showed that the genome is a double-stranded DNA and about 34kb in size. The sequenced genomic fragments showed highly similarities to gp04 and gp16 sequence of other <i>Myoviridae</i> bacteriophages at protein level.</p>","PeriodicalId":39121,"journal":{"name":"Open Biomedical Engineering Journal","volume":"9 ","pages":"185-187"},"PeriodicalIF":0.0000,"publicationDate":"2015-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5396062/pdf/","citationCount":"2","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Open Biomedical Engineering Journal","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.2174/1874120701509010185","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2015/1/1 0:00:00","PubModel":"eCollection","JCR":"Q3","JCRName":"Medicine","Score":null,"Total":0}
引用次数: 2
Abstract
An Aeromonas punctata bacteriophage, named as DH1, was isolated from East Lake, Wuhan city, China. Morphologically, phage DH1 showed a typical Myoviridae structure consisting of an isometric head (50 nm in diameter) and a visible tail. The bacteriophage had a latent period of about 90 minutes and an average burst size of about 125 PFU•Cell-1. Restriction enzyme pattern of the bacteriophage's genome showed that the genome is a double-stranded DNA and about 34kb in size. The sequenced genomic fragments showed highly similarities to gp04 and gp16 sequence of other Myoviridae bacteriophages at protein level.
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