RNA-seq analysis of mangosteen (Garcinia mangostana L.) fruit ripening

Azhani Abdul-Rahman, Hoe-Han Goh, Kok-Keong Loke, Normah Mohd Noor, Wan Mohd Aizat
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引用次数: 13

Abstract

Mangosteen (Garcinia mangostana L.) is known for its delectable taste and contains high amount of xanthones which have been reported to possess anti-cancer, anti-inflammatory and other bioactive properties. However, stage-specific regulation of mangosteen fruit ripening has never been studied in detail. We have performed a comparative transcriptomic analysis of three ripening stages (Stage 0, 2 and 6) of mangosteen. We have obtained a raw data from six libraries through Illumina HiSeq 4000. A total of ~ 40 Gb of raw data were generated. Clean reads of 650,887,650 (bp) were obtained from 656,913,570 (bp) raw reads. The raw transcriptome data were deposited to SRA database, with the BioProject accession number of PRJNA339916. These data will be beneficial for transcriptome profiling in order to study the regulation of mangosteen fruit ripening. The lack of a complete sequence database from this species impedes protein identification. These data sets provide a reference data for the exploration of novel genes or proteins to understand mangosteen fruit ripening behaviour.

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山竹果实成熟的RNA-seq分析
山竹果(Garcinia mangostana L.)以其美味的味道和含有大量的山酮而闻名,据报道,山酮具有抗癌、抗炎和其他生物活性。然而,山竹果实成熟的阶段特异性调控从未被详细研究过。我们对山竹的三个成熟期(0、2和6期)进行了比较转录组学分析。我们通过Illumina HiSeq 4000获得了6个文库的原始数据。总共产生了~ 40gb的原始数据。从656,913,570 (bp) raw reads中获得650,887,650 (bp)的Clean reads。原始转录组数据存入SRA数据库,BioProject登录号为PRJNA339916。这些数据将有利于转录组分析,以研究山竹果实成熟的调控。该物种缺乏完整的序列数据库,阻碍了蛋白质鉴定。这些数据集为探索新的基因或蛋白质来了解山竹果实的成熟行为提供了参考数据。
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