Molecular typing of human platelet antigens in immune thrombocytopenia patients in northern Brazil

Revista Brasileira de Hematologia e Hemoterapia Pub Date : 2017-04-01 DOI:10.1016/j.bjhh.2017.01.003

Julia Cavalcante do Carmo , Prissyla de Souza Klippel , Sabrine da Costa Cordeiro , Ângela Maria dos Santos Fernandes , Raquel Medeiros Pinto , Simone Schneider Weber , Cleiton Fantin

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引用次数: 5

Abstract

Background

Immune thrombocytopenia is an immune disease characterized by thrombocytopenia and bleeding due to platelet antibodies against platelet membrane glycoproteins. Human platelet antigens are derived from polymorphisms of these glycoproteins. The aim of this study was to investigate human platelet antigen frequencies in immune thrombocytopenia patients from the state of Amazonas, Brazil and investigate the potential association between specific antigens and risk for immune thrombocytopenia.

Method

Human platelet antigen typing was performed by BeadChip technology to determine allelic variants of 11 systems (HPA-1 to HPA-9, HPA-11 and HPA-15). Thirty-six patients (8 male and 28 female) with a median age of 34 years (range: 9–69 years) were evaluated and compared with data from Amazonas blood donors.

Results

Platelet counts varied from 3 to 98 × 10⁹/L. The allele frequencies were 0.944 for HPA-1a, 0.056 for HPA-1b, 0.847 for HPA-2a, 0.153 for HPA-2b, 0.555 for HPA-3a, 0.444 for HPA-3b, 0.805 for HPA-5a, 0.222 for HPA-5b, 0.9975 for HPA-9a, 0.025 for HPA-9b, 0.486 for HPA-15a and 0.513 for HPA-15b. Among immune thrombocytopenia individuals, no b allele of the HPA-4, -6, -7, -8 and -11 were found.

Conclusions

The results suggest HPA-1a, HPA-3b and HPA-5b are immune thrombocytopenia-specific autoepitopes.

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巴西北部免疫性血小板减少症患者血小板抗原的分子分型

背景免疫性血小板减少症是一种以血小板减少和血小板膜糖蛋白抗体引起的出血为特征的免疫性疾病。人血小板抗原来源于这些糖蛋白的多态性。本研究的目的是调查来自巴西亚马逊州的免疫性血小板减少症患者的血小板抗原频率，并调查特定抗原与免疫性血小板减少症风险之间的潜在关联。方法采用BeadChip技术进行人血小板抗原分型，检测11个系统(HPA-1 ~ HPA-9、HPA-11和HPA-15)的等位基因变异。36例患者(男性8例，女性28例)的平均年龄为34岁(范围:9-69岁)，并与亚马逊献血者的数据进行了比较。结果血小板计数范围为3 ~ 98 × 109/L。等位基因频率分别为:HPA-1a 0.944、HPA-1b 0.056、HPA-2a 0.847、HPA-2b 0.153、HPA-3a 0.555、HPA-3b 0.444、HPA-5a 0.805、HPA-5b 0.222、HPA-9a 0.9975、HPA-9b 0.025、HPA-15a 0.486、HPA-15b 0.513。在免疫性血小板减少症患者中，未发现HPA-4、-6、-7、-8和-11等位基因b。结论HPA-1a、HPA-3b和HPA-5b是免疫血小板减少特异性自身表位。

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来源期刊

Revista Brasileira de Hematologia e Hemoterapia

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审稿时长

21 weeks

期刊介绍： A Revista Brasileira de Hematologia e Hemoterapia é um periódico científico de propriedade da Associação Brasileira de Hematologia e Hemoterapia, publicada bimestralmente. A abreviatura de seu título é Rev. Bras. Hematol. Hemoter., que deve ser usada em bibliografias, notas de rodapé e em referências e legendas bibliográficas.