Study of Testicular Structure in Fetuses with Prune Belly Syndrome.

IF 1.8 Q3 UROLOGY & NEPHROLOGY Advances in Urology Pub Date : 2017-01-01 Epub Date: 2017-05-21 DOI:10.1155/2017/3254980

Luciano A Favorito, Suelen F Costa, Waldemar S Costa, Rodrigo Vieiralves, Fabio O Bernardo, Francisco J B Sampaio

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引用次数: 7

Abstract

Purpose: To compare the structure of the testis in fetuses with prune belly syndrome (PBS) to normal controls.

Materials and methods: We studied 6 testes obtained from 3 fetuses with PBS and 14 testes from 7 male fetuses. The testicular specimens were cut into 5-μm thick sections and stained with hematoxylin and eosin (HE), to observe the seminiferous tubules; Weigert's solution to observe elastic fibers; and picrosirius red to observe collagen. The images were captured with an Olympus BX51 microscope and Olympus DP70 camera. The stereological analysis was done with the Image Pro and Image J programs. Means were statistically compared using the Mann-Whitney U test (p < 0.005).

Results: Quantitative analysis documented no differences (p = 0.4) in number of seminiferous tubules (ST) in PBS testes (mean = 8.87%, SD = 1.59), when compared to the control (mean = 11.4%, SD = 2.99) and no differences (p = 0.8) in diameter of ST in PBS testes (mean = 52.85 μm, SD = 1.58) when compared to the control group (mean = 53.17 μm, SD = 1.55), but we did observe a lower number (p = 0.0002) of Leydig cells in the PBS testes (mean = 67.03% and SD = 3.697) when compared to the control group (mean = 90.1% and SD = 2.986).

Conclusions: Our study showed a lower concentration of Leydig cells in the triad syndrome fetuses.

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梅干腹综合征胎儿睾丸结构的研究。

目的:比较梅干腹综合征(PBS)胎儿与正常对照组的睾丸结构。材料和方法:我们研究了3例PBS胎儿的6个睾丸和7例男性胎儿的14个睾丸。取睾丸标本切成5 μm厚的切片，苏木精和伊红(HE)染色，观察精小管;威格特溶液观察弹性纤维;并以小天狼星红观察胶原蛋白。图像由奥林巴斯BX51显微镜和奥林巴斯DP70相机拍摄。利用Image Pro和Image J程序进行立体分析。均值比较采用Mann-Whitney U检验(p < 0.005)。结果:定量分析显示，PBS组睾丸精管数量(平均8.87%，SD = 1.59)与对照组(平均11.4%，SD = 2.99)比较，差异无统计学意义(p = 0.4); PBS组睾丸精管直径(平均52.85 μm, SD = 1.58)与对照组(平均53.17 μm, SD = 1.55)比较，差异无统计学意义(p = 0.8);但我们确实观察到PBS组睾丸间质细胞数量(平均67.03%，SD = 3.697)低于对照组(平均90.1%，SD = 2.986)， p = 0.0002。结论:我们的研究表明，三联症胎儿的间质细胞浓度较低。

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来源期刊

Advances in Urology UROLOGY & NEPHROLOGY-

CiteScore

2.90

自引率

0.00%

发文量

审稿时长

15 weeks

期刊介绍： Advances in Urology is a peer-reviewed, open access journal that publishes state-of-the-art reviews and original research papers of wide interest in all fields of urology. The journal strives to provide publication of important manuscripts to the widest possible audience worldwide, without the constraints of expensive, hard-to-access, traditional bound journals. Advances in Urology is designed to improve publication access of both well-established urologic scientists and less well-established writers, by allowing interested scientists worldwide to participate fully.