Arbuscular Mycorrhizal Fungi Increase the Phenolic Compounds Concentration in the Bark of the Stem of Libidibia Ferrea in Field Conditions.

Abstract

Background: Libidibia ferrea is a species particular to the caatinga presenting medicinal properties for containing bioactive compounds. The use of Arbuscular Mycorrhizal Fungi (AMF) can increase the production of biomolecules in the legume leaves; however, no light has been shed on the role of symbiosis in maximizing metabolites production in the bark of L. ferrea stem.

Objective: The aim was to select AMF that are efficient at increasing the production of phenolic compounds with medicinal properties in the bark of the L. ferrea stem.

Methods: The experiment was designed in randomized blocks with four inoculation treatments (plants pre-inoculated with Claroideoglomus etunicatum, with Gigaspora albida, with Acaulospora longula, and non-inoculated plants - control) with six repetitions. Thirteen months after the transplanting, the plants were pruned and the bark of the stem was collected; subsequently, this plant material was dried in a chamber. After the drying process, fractions of the bark of the stem were macerated in methanol. The extracts were further used for analyses of the biomolecules.

Results: The flavonoids concentration had an increase of, respectively, 236% and 186% in relation to the control for the treatments with A. longula and C. etunicatum; plants inoculated with A. longula had an increase of 47% in total tannins concentration compared with the non-inoculated control - a benefit that the proanthocyanidins did not present.

Conclusion: Applying inoculation with A. longula may be an alternative to increase the production of biomolecules of the secondary metabolism in the bark of the L. ferrea stem in field conditions.