{"title":"Aging and hydrocortisone effects on transient structures of replicative DNA of human fibroblasts.","authors":"C Icard-Liepkalns, A Macieira-Coelho","doi":"10.3181/00379727-170-41445","DOIUrl":null,"url":null,"abstract":"Abstract Alkali-labile sites accumulate in DNA during aging in vitro of human embryonic lung fibroblasts. The sedimentation velocity of newly synthesized DNA was analyzed in alkaline sucrose gradients in young and old cells. Although most of the old cells had gone through a whole S period, their DNA sedimented in a dispersed fashion. Hydrocortisone shortened the time of appearance of bulk DNA. The results are compatible with the slowdown of DNA chain elongation during aging and could explain to a certain extent the sustaining effect of hydrocortisone on the human fibroblast lifespan.","PeriodicalId":20675,"journal":{"name":"Proceedings of the Society for Experimental Biology and Medicine","volume":" ","pages":"373-7"},"PeriodicalIF":0.0000,"publicationDate":"1982-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3181/00379727-170-41445","citationCount":"4","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Proceedings of the Society for Experimental Biology and Medicine","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.3181/00379727-170-41445","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 4
Abstract
Abstract Alkali-labile sites accumulate in DNA during aging in vitro of human embryonic lung fibroblasts. The sedimentation velocity of newly synthesized DNA was analyzed in alkaline sucrose gradients in young and old cells. Although most of the old cells had gone through a whole S period, their DNA sedimented in a dispersed fashion. Hydrocortisone shortened the time of appearance of bulk DNA. The results are compatible with the slowdown of DNA chain elongation during aging and could explain to a certain extent the sustaining effect of hydrocortisone on the human fibroblast lifespan.
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